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Plant Cell, Published on June 13, 2008; 10.1105/tpc.108.060541 Arabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence Motifs

Abstract

Dong Wook Lee,^a,1 Jong Kyoung Kim,^b,1 Sumin Lee,^a Seungjin Choi,^b Sanguk Kim,^c,2 and Inhwan Hwang^a,2

^a Laboratory of Cellular Systems Biology, Division of Molecular and Life Sciences, POSTECH, Pohang 790-784, Korea
^b Department of Computer Science, POSTECH, Pohang 790-784, Korea
^c Laboratory of Structural Bioinformatics, Division of Molecular and Life Sciences, POSTECH, Pohang 790-784, Korea

The N-terminal transit peptides of nuclear-encoded plastidproteins are necessary and sufficient for their import intoplastids, but the information encoded by these transit peptidesremains elusive, as they have a high sequence diversity andlack consensus sequences or common sequence motifs. Here, weinvestigated the sequence information contained in transit peptides.Hierarchical clustering on transit peptides of 208 plastid proteinsshowed that the transit peptide sequences are grouped to multiplesequence subgroups. We selected representative proteins fromseven of these multiple subgroups and confirmed that their transitpeptide sequences are highly dissimilar. Protein import experimentsrevealed that each protein contained transit peptide-specificsequence motifs critical for protein import into chloroplasts.Bioinformatics analysis identified sequence motifs that wereconserved among members of the identified subgroups. The sequencemotifs identified by the two independent approaches were nearlyidentical or significantly overlapped. Furthermore, the accuracyof predicting a chloroplast protein was greatly increased bygrouping the transit peptides into multiple sequence subgroups.Based on these data, we propose that the transit peptides arecomposed of multiple sequence subgroups that contain distinctivesequence motifs for chloroplast targeting.

¹ These authors contributed equally to this work.
² Address correspondence to Sanguk Kim or Inhwan Hwang.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Inhwan Hwang.

논문정보

형식Research article
게재일2008년 06월 (BRIC 등록일 )
연구진국내 연구진
분야 생명과학 > 유전학

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