구.농수식품
- 조회866
Abstract
Mohammed Dwidar, Hyochan Jang, Naseer Sangwan, Wonsik Mun, Hansol Im, Sora Yoon, Sooin Choi, Dougu Nam, Robert J Mitchell
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH, 44195, USA
Mohammed Dwidar & Naseer Sangwan
Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195, USA
Mohammed Dwidar & Naseer Sangwan
Center for Microbiome and Human Health, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195, USA
Mohammed Dwidar & Naseer Sangwan
School of Life Sciences, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Ulsan, 44919, Republic of Korea
Hyochan Jang, Wonsik Mun, Hansol Im, Sora Yoon, Sooin Choi, Dougu Nam & Robert J. Mitchell
Correspondence to Mohammed Dwidar or Dougu Nam or Robert J. Mitchell.
Abstract
Bdellovibrio bacteriovorus 109J is a predatory bacterium which lives by predating on other Gram-negative bacteria to obtain the nutrients it needs for replication and survival. Here, we evaluated the effects two classes of bacterial signaling molecules (acyl homoserine lactones (AHLs) and diffusible signaling factor (DSF)) have on B. bacteriovorus 109J behavior and viability. While AHLs had a non-significant impact on predation rates, DSF considerably delayed predation and bdelloplast lysis. Subsequent experiments showed that 50 μM DSF also reduced the motility of attack-phase B. bacteriovorus 109J cells by 50% (38.2 ± 14.9 vs. 17 ± 8.9 μm/s). Transcriptomic analyses found that DSF caused genome-wide changes in B. bacteriovorus 109J gene expression patterns during both the attack and intraperiplasmic phases, including the significant downregulation of the flagellum assembly genes and numerous serine protease genes. While the former accounts for the reduced speeds observed, the latter was confirmed experimentally with 50 μM DSF completely blocking protease secretion from attack-phase cells. Additional experiments found that 30% of the total cellular ATP was released into the supernatant when B. bacteriovorus 109J was exposed to 200 μM DSF, implying that this QS molecule negatively impacts membrane integrity.
논문정보
- Research article
- 2021년 02월 (BRIC 등록일 2021-02-04)
- 국내(교신)+국외 연구진
- 생명과학 > 미생물학
- 구・농수식품
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