한빛사논문
- 조회1,205
연세대학교
Sungjin Min†,#, Hyo-Jung Lee‡,#, Yoonhee Jin†, Yu Heun Kim†, Jaesuk Sung‡, Heon-Jin Choi‡,*, Seung-Woo Cho†,§,┴,*
†Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
‡Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea
§Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea
┴Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea
#These authors contributed equally to this work.
*Corresponding authors
Abstract
Direct reprogramming is an efficient strategy to produce cardiac lineage cells necessary for cardiac tissue engineering and drug testing for cardiac toxicity. However, functional maturation of reprogrammed cardiomyocytes, which is of great importance for their regenerative potential and drug response, still remains challenging. In this study, we propose a novel electrode platform to promote direct cardiac reprogramming and improve the functionality of reprogrammed cardiac cells. Nonviral cardiac reprogramming was improved via a three-dimensional spheroid culture of chemically induced cardiomyocytes exposed to a small-molecule cocktail. A micropillar electrode array providing biphasic electrical pulses mimicking the heartbeat further enhanced maturation and electrophysiological properties of reprogrammed cardiac spheroids, leading to proper responses and increased sensitivity to drugs. On the basis of our results, we conclude that our device may have a wider application in the generation of functional cardiac cells for regenerative medicine and screening of novel drugs.
논문정보
- Research article
- 2020년 09월 (BRIC 등록일 2020-09-09)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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