한빛사논문
Minji Lee1,7, Eunmin Lee2,7, Seong Kyu Han3,7, Yoon Ha Choi2, Dong-il Kwon1, Hyobeen Choi3, Kwanghwan Lee3, Eun Seo Park2, Min-Seok Rha4, Dong Jin Joo5,6, Eui-Cheol Shin4, Sanguk Kim3,*, Jong Kyoung Kim2,* & You Jeong Lee1,*
1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
2Department of New Biology, DGIST, Daegu 42988, Republic of Korea.
3Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
4Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, Korea.
5Department of Surgery, Yonsei University, College of Medicine, Seoul, Republic of Korea.
6The Research Institute for Transplantation, Yonsei University, College of Medicine, Seoul, Republic of Korea.
7These authors equally contributed: Minji Lee, Eunmin Lee, Seong Kyu Han.
*Corresponding author
Abstract
Invariant natural killer T (iNKT), mucosal-associated invariant T (MAIT), and γδ T cells are innate T cells that acquire memory phenotype in the thymus and share similar biological characteristics. However, how their effector differentiation is developmentally regulated is still unclear. Here, we identify analogous effector subsets of these three innate T cell types in the thymus that share transcriptional profiles. Using single-cell RNA sequencing, we show that iNKT, MAIT and γδ T cells mature via shared, branched differentiation rather than linear maturation or TCR-mediated instruction. Simultaneous TCR clonotyping analysis reveals that thymic maturation of all three types is accompanied by clonal selection and expansion. Analyses of mice deficient of TBET, GATA3 or RORγt and additional in vivo experiments corroborate the predicted differentiation paths, while human innate T cells from liver samples display similar features. Collectively, our data indicate that innate T cells share effector differentiation processes in the thymus.
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TOP52020년 후보
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