박찬흠 (한림대학교 의과대학, 춘천성심병원 이비인후과) | 한빛사논문 > 한빛사

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한림대학교 의과대학, 춘천성심병원 이비인후과

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Biomaterials, Available online 12 August 2020, 120281 | https://doi.org/10.1016/j.biomaterials.2020.120281 4D-bioprinted silk hydrogels for tissue engineering

Soon Hee Kim^a, Ye Been Seo^a, Yeung Kyu Yeon^a, Young Jin Lee^a, Hae Sang Park^b, Md Tipu Sultan^a, Jung Min Lee^a, Ji Seung Lee^a, Ok Joo Lee^a, Heesun Hong^a, Hanna Lee^a, Olatunji Ajiteru^a, Ye Ji Suh^a, Sung-Hyuk Song^c, Kwang-Ho Lee^d, Chan Hum Park^a,b,*

^aNano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea
^bDepartment of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, 24252, Republic of Korea
^cDepartment of Robotics & Mechatronics, Korea Institute of Machinery & Materials, Daejeon, 34103, Republic of Korea
^dDepartment of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University, Chuncheon, 2434, Republic of Korea

^*Corresponding author

Abstract

Recently, four-dimensional (4D) printing is emerging as the next-generation biofabrication technology. However, current 4D bioprinting lacks biocompatibility or multi-component printability. In addition, suitable implantable targets capable of applying 4D bioprinted products have not yet been established, except theoretical and in vitro study. Herein, we describe a cell-friendly and biocompatible 4D bioprinting system including more than two cell types based on digital light processing (DLP) and photocurable silk fibroin (Sil-MA) hydrogel. The shape changes of 3D printed bilayered Sil-MA hydrogels were controlled by modulating their interior or exterior properties in physiological conditions. We used finite element analysis (FEA) simulations to explore the possible changes in the complex structure. Finally, we made trachea mimetic tissue with two cell types using this 4D bioprinting system and implanted it into a damaged trachea of rabbit for 8 weeks. The implants were integrated with the host trachea naturally, and both epithelium and cartilage were formed at the predicted sites. These findings demonstrate that 4D bioprinting system could make tissue mimetic scaffold biologically and suggest the potential value of the 4D bioprinting system for tissue engineering and the clinical application.

Keywords : 4D bioprinting; Digital light processing; Silk fibroin; Osmotic pressure; Trachea; Heterogenous tissue

논문정보

형식Research article
게재일2020년 08월 (BRIC 등록일 2020-08-28)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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