함승주 (연세대학교) | 한빛사논문 > 한빛사

한빛사논문

조회1,133

연세대학교

CV File 343 kb

Biosens. Bioelectron., Volume 162, 15 August 2020, 112254 | https://doi.org/10.1016/j.bios.2020.112254 Matrix metalloproteinase 9-activatable peptide-conjugated hydrogel-based fluorogenic intraocular-lens sensor

Moo-Kwang Shin^a,1, Yong Woo Ji^b,c,1, Chae-Eun Moon^b, Hyo Lee^a, Byunghoon Kang^a, Woo-Seok Jinn^a, Jisun Ki^a, Byunggeol Mun^a, Myeong-Hoon Kim^a, Hyung Keun Lee^b,d,e,*, Seungjoo Haam^a,*

^aDepartment of Chemical & Biomolecular Engineering, College of Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea
^bInstitute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea
^cDepartment of Ophthalmology, National Health Insurance Service Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang, 10444, Republic of Korea
^dInstitute of Vascular Disease and Metabolism, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea
^eCollege of Pharmacy, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon, 21983, Republic of Korea

¹These authors contributed equally to this work.

^*Corresponding author.

Abstract

The eye is an extension of the central nervous system (CNS) and contains aqueous humor (AH), which is a fluid rich in biomolecules secreted from intraocular tissues; thus, this organ allows for non-invasive visualization of early changes in CNS disorders. There is a growing interest in developing implantable devices, such as intraocular-lens (IOL), for specific medical uses, including intraocular monitoring. We describe a novel IOL-sensing system for detecting AH biomarkers via biocompatible enzyme-activatable fluorogenic hydrogel sensors. Matrix-metalloproteinase-9, a biomarker of degenerative CNS and eye disorders, was selected as a target. A peptide-probe-incorporated fluorogenic IOL (FIOL) was developed using diacrylamide-group-modified poly(ethyleneglycol) (PEGDAAm) biocompatible hydrogels, adjusting the hydrogel mesh size to allow selective penetration of the target while blocking non-targets, using label-free detection with semi-permanently implantable sensors, and demonstrating the clinical feasibility of FIOL through in vivo testing. This novel FIOL-based sensing system represents a promising approach for liquid biopsy of intraocular fluids.

Keywords : Intraocular-lens sensor; Hydrogel sensor; Peptide-probe; Matrix metalloproteinase-9; Liquid biopsy

논문정보

형식Research article
게재일2020년 08월 (BRIC 등록일 2020-08-21)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기