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Biosens. Bioelectron., Available online 15 August 2020, 112514 | https://doi.org/10.1016/j.bios.2020.112514 High-performance portable graphene field-effect transistor device for detecting gram-positive and -negative bacteria

Kyung Ho Kim^a,1, Seon Joo Park^a,1, Chul Soon Park^a, Sung Eun Seo^a, Jiyeon Lee^a, Jinyeong Kim^a, Seung Hwan Lee^b, Soohyun Lee^a, Jun-Seob Kim^a, Choong-Min Ryu^a, Dongeun Yong^c, Hyeonseok Yoon^d, Hyun Seok Song^e, Sang Hun Lee^f,*, Oh Seok Kwon^a,g,*

^aInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
^bDepartment of Bionano Engineering, Hanyang University, Ansan, Republic of Korea
^cDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Republic of Korea
^dSchool of Polymer Science and Engineering and Department of Polymer Engineering, Graduate School, Chonnam National University, Gwangju, 61186, Republic of Korea
^eSensor System Research Center, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea
^fDepartment of Bioengineering, University of California Berkeley, CA, 94720, USA
^gDepartment of Nanobiotechnology, Korea University of Science and Technology (UST), Republic of Korea

¹These authors contributed equally to this work.

^*Corresponding author

Abstract

Current techniques for Gram-typing and for diagnosing a pathogen at the early infection stage rely on Gram stains, cultures, Enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and gene microarrays, which are labor-intensive and time-consuming approaches. In addition, a delayed or imprecise diagnosis of clinical pathogenic bacteria leads to a life-threatening emergency or overuse of antibiotics and a high-rate occurrence of antimicrobial-resistance microbes. Herein, we report high-performance antibiotics (as bioprobes) conjugated graphene micropattern field-effect transistors (ABX-GMFETs) to facilitate on-site Gram-typing and help in the detection of the presence or absence of Gram-negative and -positive bacteria in the samples. The ABX-GMFET platform, which consists of recognition probes and GM transistors conjugated with novel interfacing chemical compounds, was integrated into the microfluidics to minimize the required human intervention and facilitate automation. The mechanism of binding of ABX-GMFET was based on a charge or chemical moiety interaction between the bioprobes and target bacteria. Subsequently, ABX-GMFETs exhibited unprecedented high sensitivity with a limit of detection (LOD) of 100 CFU/mL (1–9 CFU/mL), real-time target specificity.

Keywords : Portable biosensor; Graphene field-effect transistor; Interfacing chemistry; Microfluidics; Real-time monitoring; Bioprobes

논문정보

형식Research article
게재일2020년 08월 (BRIC 등록일 2020-08-25)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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