상위피인용논문
- 조회501
Minjoung Jo,1,* Ji-Young Ahn,1,* Joohyung Lee,2,* Seram Lee,1 Sun Woo Hong,3 Jae-Wook Yoo,3 Jeehye Kang,1 Pooja Dua,1 Dong-ki Lee,3 Seunghun Hong,2 and Soyoun Kim1
1Department of Biomedical Engineering, Dongguk University, Seoul, Korea.
2Department of Physics, and Department of Biophysics and Chemical Biology, Seoul National University, Seoul, Korea.
3Department of Chemistry, Sungkyunkwan University, Suwon, Korea.
*These three authors contributed equally to this work.
Address correspondence to: Prof. Dong-ki Lee, Dr. Seunghun Hong, Prof. Soyoun Kim
Abstract
The development of reagents with high affinity and specificity to small molecules is crucial for the high-throughput detection of chemical compounds, such as toxicants or pollutants. Aptamers are short and single-stranded (ss) oligonucleotides able to recognize target molecules with high affinity. Here, we report the selection of ssDNA aptamers that bind to Bisphenol A (BPA), an environmental hormone. Using SELEX process, we isolated high affinity aptamers to BPA from a 1015 random library of 60 mer ssDNAs. The selected aptamers bound specifically to BPA, but not to structurally similar molecules, such as Bisphenol B with one methyl group difference, or 4,4′-Bisphenol with 2 methyl groups difference. Using these aptamers, we developed an aptamer-based sol–gel biochip and detected BPA dissolved in water. This novel BPA aptamer-based detection can be further applied to the universal and high-specificity detection of small molecules.
논문정보
- Research article
- 2011년 04월 (BRIC 등록일 2020-12-29)
- 국내 연구진
- 120회 이상 인용된 논문
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