한빛사논문
- 조회843
Rongjia Shia,1, Shuaihui Fenga,1, Chan Yeong Parka,1, Kyung Yeol Parka, Jeonghun Songb, Jong Pil Parkb, Hyang Sook Chunb, Tae Jung Parka,*
aDepartment of Chemistry, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea
bDepartment of Food Science and Biotechnology, School of Food Science and Technology, Chung-Ang University, 4726 Seodong-daero, Anseong 17546, Republic of Korea
1These authors equally contributed to this work.
*Corresponding author.
Abstract
Histamine is primarily found in spoiled food and often used as an indicator of food safety. Compared to various existing methods for analyzing histamine, high-performance liquid chromatography (HPLC) which is accurate but time-consuming, and immunochemical methods that are difficult to produce high specificity and affinity antibodies towards small molecules have been used. In this study, we developed a newly designed, sensitive, and selective fluorescence detection platform for histamine sensing, utilizing carbon quantum dots (CQDs) and synthetic peptides. Specifically, through biopanning approaches, a series of peptides having a high affinity towards immobilized histamine hapten were selected from phage-displayed libraries. Then, CQDs were synthesized by one-pot hydrothermal treatment enabling their fluorescence to be effectively quenched by peptides via the electron transfer interactions. While, in the presence of histamine, fluorescence will be recovered because of the stronger interaction between peptide and target. In this study, from the selectivity tests towards histamine and in contrast to structurally similar compounds, peptide Hisp3 (DIDRAGKASHWP) along with its dipeptide repeat derivative (Hisp3-2-C) were chemically synthesized to be used as promising histamine receptors. Furthermore, the application of peptide along with gold-coated magnetic nanoparticles (MNP@Au NPs) was designed for purification and analysis of fish samples. These results indicate that the CQDs and peptide sensor system could detect histamine at lower concentrations with high sensitivity and selectivity.
Keywords : Histamine; Carbon quantum dots; Phage display; Specific peptide bioreceptor; Fluorescent sensing
논문정보
- Research article
- 2020년 08월 (BRIC 등록일 2020-08-24)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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