한빛사논문
- 조회879
David A. Hastman1,2, Joseph S. Melinger3, Guillermo Lasarte-Aragonés1,4,5, Paul D. Cunningham3, Matthew C. Chiriboga1,6, Zachary J. Salvato1, Thomas M. Salvato1, Carl W. Brown III1,4, Divita Mathur1,4, Igor L. Medintz1, Eunkeu Oh7,* and Sebastián A. Díaz1,*
1Center for Bio/Molecular Science and Engineering, Code 6900
3Electronics Science and Technology Division, Code 6800
7Division of Optical Science, Code 5600, U.S. Naval Research Laboratory, Washington, DC 20375 USA
2Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA
4College of Science
6Department of Bioengineering, George Mason University, Fairfax, VA 22030 USA
5Current Address: Analytical Chemistry Department, University of Córdoba, 14071-Córdoba (Spain)
*To whom correspondence should be addressed.
Abstract
Femtosecond (fs) laser pulsed excitation of plasmonic nanoparticle (NP)–biomolecule conjugates is a promising method to locally heat biological materials. Studies have demonstrated that fs pulses of light can modulate the activity of DNA or proteins when attached to plasmonic NPs; however, the precision over subsequent biological function remains largely undetermined. Specifically, the temperature the localized biomolecules “experience” remains unknown. We used 55 nm gold nanoparticles (AuNPs) displaying double-stranded (ds) DNA to examine how, for dsDNA with different melting temperatures, the laser pulse energy fluence and bulk solution temperature affect the rate of local DNA denaturation. A universal “template” single-stranded DNA was attached to the AuNP surface, and three dye-labeled probe strands, distinct in length and melting temperature, were hybridized to it creating three individual dsDNA-AuNP bioconjugates. The dye-labeled probe strands were used to quantify the rate and amount of DNA release after a given number of light pulses, which was then correlated to the dsDNA denaturation temperature, resulting in a quantitative nanothermometer. The localized DNA denaturation rate could be modulated by more than threefold over the biologically relevant range of 8–53 °C by varying pulse energy fluence, DNA melting temperature, and surrounding bath temperature. With a modified dissociation equation tailored for this system, a “sensed” temperature parameter was extracted and compared to simulated AuNP temperature profiles. Determining actual biological responses in such systems can allow researchers to design precision nanoscale photothermal heating sources.
KEYWORDS:plasmonic nanoparticles, photothermal effect, local heating, femtosecond pulsed laser, gold nanoparticle, DNA denaturation
논문정보
- Research article
- 2020년 07월 (BRIC 등록일 2020-07-29)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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