방두희 (연세대학교) | 한빛사논문 > 한빛사

상위피인용논문

조회1,161

연세대학교

CV File 182 kb

Exp. Mol. Med., Volume 50, Issue 8, 1 August 2018, Article number 96 | https://doi.org/10.1038/s12276-018-0071-8 Single-cell RNA sequencing technologies and bioinformatics pipelines

Byungjin Hwang¹, Ji Hyun Lee^2,3,* and Duhee Bang^1,*

¹ Department of Chemistry, Yonsei University, Seoul, Korea.
² Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Korea.
³ Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, Korea.

^*Corresponding author

Abstract

Rapid progress in the development of next-generation sequencing (NGS) technologies in recent years has provided many valuable insights into complex biological systems, ranging from cancer genomics to diverse microbial communities. NGS-based technologies for genomics, transcriptomics, and epigenomics are now increasingly focused on the characterization of individual cells. These single-cell analyses will allow researchers to uncover new and potentially unexpected biological discoveries relative to traditional profiling methods that assess bulk populations. Single-cell RNA sequencing (scRNA-seq), for example, can reveal complex and rare cell populations, uncover regulatory relationships between genes, and track the trajectories of distinct cell lineages in development. In this review, we will focus on technical challenges in single-cell isolation and library preparation and on computational analysis pipelines available for analyzing scRNA-seq data. Further technical improvements at the level of molecular and cell biology and in available bioinformatics tools will greatly facilitate both the basic science and medical applications of these sequencing technologies.

논문정보

형식Review Paper
게재일2018년 08월 (BRIC 등록일 2020-07-16)
연구진국내 연구진
분야 의약학 > 유전 및 유전체의학
피인용횟수60회 이상 인용된 논문

댓글 작성 로그인

CV 열람하기

연락처 보기