구.농수식품
- 조회935
Abstract
Hye‐Ryung Kim1, Da‐Rae Lim1,2, Ha‐Gyeong Chae1,2, Ji‐Yong Park2, Seong‐Hee Kim2, Kyoung‐Ki Lee2, Changhee Lee3, Young S. Lyoo4, Choi‐Kyu Park1,*
1College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Republic of Korea.
2Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
3Animal Virology Laboratory, School of Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
4College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea
*Correspondence
Summary
Porcine circovirus type 3 (PCV3) is an emerging viral pathogen that has been identified in pigs with various clinical signs. For rapid and specific detection of PCV3, an advanced real‐time loop‐mediated isothermal amplification (rLAMP) assay that uses both assimilating probes and swarm primers was developed and evaluated in this study. The assay specifically amplified PCV3 DNA, but it did not amplify other porcine viral nucleic acids. The limit of detection of rLAMP with swarm primers was 50 PCV3 DNA copies/reaction, which was comparable to that of the real‐time quantitative polymerase chain reaction (qPCR) and 10 times more sensitive than rLAMP without swarm primers. In an evaluation of clinical samples, the rLAMP assay was able to detect PCV3 DNA within 17.34 ± 4.45 min, which is more rapid than what has been previously reported for the standard qPCR assay (31.78 ± 4.60 min). Detection with rLAMP was largely in agreement with that of the qPCR with a kappa value (95% confidence interval) of 0.98 (0.95–1.00). Taken together, these results suggest that the rLAMP assay presented will be a valuable tool for rapid, specific, and reliable diagnosis of PCV3 in clinical samples.
Keywords : Assimilating probe, Porcine circovirus 3, Real-time LAMP, Swarm primer
논문정보
- Research article
- 2020년 06월 (BRIC 등록일 2020-07-08)
- 국내 연구진
- 구・농수식품
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