구.농수식품
- 조회1,316
Abstract
Keesun Yu1, Min Jeong Gu1, Young Jin Pyung1, Ki‑Duk Song2, Tae Sub Park3, Seung Hyun Han4 and Cheol‑Heui Yun1,3,5,*
1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. 2 Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University, Jeonju 54896, Republic of Korea. 3 Graduate School of International Agricultural Technology and Institute of Green‑Bio Science and Technology, Seoul National University, Pyeongchang‑gun, Seoul, Gangwon‑do 25354, Republic of Korea. 4 Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Programme, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea. 5 Center for Food Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea.
*Corresponding author
Abstract
Monocytes/macrophages, which are found in a variety of organs, maintain tissue homeostasis at a steady state and act as the first line of defence during pathogen-induced inflammation in the host. Most monocyte/macrophage lineage studies in chickens have been largely performed using cell lines, while few studies using primary cells have been conducted. In the present study, the phenotypic and functional characteristics of splenic monocyte/macrophage lineage cells during steady state and inflammatory conditions were examined. Splenic monocyte/macrophage lineage cells could be identified as MRC1loMHCIIhi and MRC1hiMHCIIlo cells based on their surface expression of MRC1 and MHCII. In the steady state, MRC1loMHCIIhi cells were more frequently found among MRC1+ cells. MRC1loMHCIIhi cells expressed a higher number of antigen-presenting molecules (MHCII, MHCI, and CD80) than MRC1hiMHCIIlo cells. In contrast, MRC1hiMHCIIlo cells showed better phagocytic and CCR5-dependent migratory properties than MRC1loMHCIIhi cells. Furthermore, MRC1hiMHCIIlo cells infiltrated the spleen in vivo and then became MRC1loMHCIIhi cells. During lipopolysaccharide (LPS)-induced inflammatory conditions that were produced via intraperitoneal (i.p.) injection, the proportion and absolute number of MRC1hiMHCIIlo cells were increased in the spleen. Uniquely, inflammation induced the downregulation of MHCII expression in MRC1hiMHCIIlo cells. The major source of inflammatory cytokines (IL-1β, IL-6, and IL-12) was MRC1loMHCIIhi cells. Furthermore, MRC1hiMHCIIlo cells showed greater bactericidal activity than MRC1loMHCIIhi cells during LPS-induced inflammation. Collectively, these results suggest that two subsets of monocyte/macrophage lineage cells exist in the chicken spleen that have functional differences.
논문정보
- Research article
- 2020년 05월 (BRIC 등록일 2020-07-03)
- 국내 연구진
- 생명과학 > 동물자원학
- 구・농수식품
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