구.농수식품
- 조회914
Abstract
Ji Eun Lee1,†, Yoon‑Chul Kye1,†, Sung‑Moo Park1,2,†, Byoung‑Shik Shim3, Sungsik Yoo4, Eunmi Hwang5, Hyungkuen Kim5, Sung‑Jo Kim5, Seung Hyun Han6, Tae Sub Park7, Byung‑Chul Park7,* and Cheol‑Heui Yun1,2,7,*
1 Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. 2 Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea. 3 G+FLAS Life Sciences, Seoul, Republic of Korea. 4 Choong‑Ang Vaccine Laboratory, Daejeon, Republic of Korea. 5 Department of Biotechnology, Hoseo University, Asan, Chungcheongnam‑do, Republic of Korea. 6 Department of Oral Microbiology and Immunology, DRI and BK21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea. 7 Graduate School of International Agricultural Technology, Institute of Green‑Bio Science and Technology, Seoul National University, Pyeongchang‑gun, Gangwon‑do, Republic of Korea.
*Corresponding authors
†Ji Eun Lee, Yoon‑Chul Kye and Sung‑Moo Park contributed equally to this work
Abstract
Low-pathogenicity avian influenza H9N2 remains an endemic disease worldwide despite continuous vaccination, indicating the need for an improved vaccine strategy. Bacillus subtilis (B. subtilis), a gram-positive and endospore-forming bacterium, is a non-pathogenic species that has been used in probiotic formulations for both animals and humans. The objective of the present study was to elucidate the effect of B. subtilis spores as adjuvants in chickens administered inactivated avian influenza virus H9N2. Herein, the adjuvanticity of B. subtilis spores in chickens was demonstrated by enhancement of H9N2 virus-specific IgG responses. B. subtilis spores enhanced the proportion of B cells and the innate cell population in splenocytes from chickens administered both inactivated H9N2 and B. subtilis spores (Spore + H9N2). Furthermore, the H9N2 and spore administration induced significantly increased expression of the pro-inflammatory cytokines IL-1β and IL-6 compared to that in the H9N2 only group. Additionally, total splenocytes from chickens immunized with inactivated H9N2 in the presence or absence of B. subtilis spores were re-stimulated with inactivated H9N2. The subsequent results showed that the extent of antigen-specific CD4+ and CD8+ T cell proliferation was higher in the Spore + H9N2 group than in the group administered only H9N2. Taken together, these data demonstrate that B. subtilis spores, as adjuvants, enhance not only H9N2 virus-specific IgG but also CD4+ and CD8+ T cell responses, with an increase in pro-inflammatory cytokine production. This approach to vaccination with inactivated H9N2 together with a B. subtilis spore adjuvant in chickens produces a significant effect on antigen-specific antibody and T cell responses against avian influenza virus.
논문정보
- Research article
- 2020년 05월 (BRIC 등록일 2020-06-30)
- 국내 연구진
- 생명과학 > 동물자원학
- 구・농수식품
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