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한양대학교

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Biomaterials, Available online 12 June 2020, 120192 | https://doi.org/10.1016/j.biomaterials.2020.120192 Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering

Jinkyu Lee^a,b, Sangmin Lee^a,b, Taufiq Ahmad^a,b, Sajeesh Kumar Madhurakat Perikamana^a,b, Jinki Lee^a,b, Eun Mi Kim^a,b, Heungsoo Shin^a,b,c,*

^aDepartment of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, 04763, Republic of Korea
^bBK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 04763, Republic of Korea
^cInstitute of Nano Science and Technology, Hanyang University, Seoul, 04763, Republic of Korea

^*Corresponding author

Abstract

Stem cells with mineralized materials have been used for bone regeneration; however, engineering the complex vascularized structure of the natural bone remains a challenge. Here, we developed platelet-derived growth factor (PDGF) and bio-mineral coated fibers which were then assembled with human adipose-derived stem cells (hADSCs) to form spheroids as building blocks for vascularized bone regeneration. The PDGF incorporated within the spheroid increased the proliferation of hADSCs, which was characterized by Ki-67 staining and DNA contents. Furthermore, the PDGF enhanced not only osteogenic differentiation, but also endothelial differentiation of hADSCs; the cells within the spheroids showed significantly greater gene expression by 2.46 ± 0.14 fold for osteocalcin (OCN) and by 12.85 ± 3.36 fold for von Willebrand factor (vWF) than those without PDGF. Finally, at two months following transplantation of PDGF-incorporated spheroids onto in vivo mouse calvarial defect, the regenerated bone area (42.48 ± 10.84%) was significantly enhanced and the greatest number of capillaries and arterioles with indication of transplanted hADSCs were observed. Moreover, millimeter-scale in vitro tissue prepared by fused assembly of the spheroids exhibited greater mRNA expression-associated to endothelial lineage. Taken together, these findings indicate that stem cell spheroids incorporating PDGF and bio-minerals could be used as a module for successful vascularized bone regeneration.

Keywords : Fragmented fibers; Spheroid; hADSCs; Stem cell; Angiogenic factor; Bone regeneration

논문정보

형식Research article
게재일2020년 06월 (BRIC 등록일 2020-06-17)
연구진국내 연구진
분야 생명과학 > 생물공학

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