한빛사논문
- 조회2,178
Jong Woo Bae1,2, S. Chul Kwon1,2, Yongwoo Na1,2, V. Narry Kim1,2,* and Jong-Seo Kim1,2,*
1Center for RNA Research, Institute for Basic Science, Seoul, Korea. 2School of Biological Sciences, Seoul National University, Seoul, Korea.
*Corresponding authors
Abstract
RNA-binding sites (RBSs) can be identified by liquid chromatography and tandem mass spectrometry analyses of the protein–RNA conjugates created by crosslinking, but RBS mapping remains highly challenging due to the complexity of the formed RNA adducts. Here, we introduce RBS-ID, a method that uses hydrofluoride to fully cleave RNA into mono-nucleosides, thereby minimizing the search space to drastically enhance coverage and to reach single amino acid resolution. Moreover, the simple mono-nucleoside adducts offer a confident and quantitative measure of direct RNA–protein interaction. Using RBS-ID, we profiled ~2,000 human RBSs and probed Streptococcus pyogenes Cas9 to discover residues important for genome editing.
논문정보
TOP52020년 선정
- Research article
- 2020년 06월 (BRIC 등록일 2020-06-11)
- 국내 연구진
- 생명과학 > 생화학
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