Sau Fung Wong (고려대학교) | 한빛사논문 > 한빛사

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Sau Fung WongSau Fung Wong

고려대학교

Biomaterials, Volume 32, Issue 32, November 2011, Pages 8087-8096 | https://doi.org/10.1016/j.biomaterials.2011.07.028 Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model

Sau Fung Wong^a, Da Yoon No^a, Yoon Young Choi^a, Dong Sik Kim^b, Bong Geun Chung^c, Sang-Hoon Lee^a,*

^aDepartment of Biomedical Engineering, Korea University, Seoul 136-701, Republic of Korea
^bDepartment of Surgery, Korea University, Seoul 136-701, Republic of Korea
^cDepartment of Bionano Engineering, Hanyang University, Ansan 436-791, Republic of Korea

^*Corresponding author

Abstract

We have developed a size-controllable spheroidal hepatosphere and heterosphere model by mono-culturing of primary hepatocytes and by co-culturing primary hepatocytes and hepatic stellate cells (HSCs). We demonstrated that uniform-sized heterospheres, which self-aggregated from primary hepatocytes and HSCs, formed within concave microwell arrays in a rapid and homogeneous manner. The effect of HSCs was quantitatively and qualitatively investigated during spheroid formation, and HSC played an important role in controlling the organization of the spheroidal aggregates and formation of tight cell–cell contacts. An analysis of the metabolic function showed that heterospheres secreted 30% more albumin than hepatospheres on day 8. In contrast, the urea secretion from heterospheres was similar to that of hepatospheres. A quantitative cytochrome P450 assay showed that the enzymatic activity of heterospheres cultured for 9 days was higher as compared with primary hepatospheres. These size-controllable heterospheres could be mass-produced using concave plate and be useful for creating artificial three-dimensional hepatic tissue constructs and regeneration of failed liver.

논문정보

형식Research article
게재일2011년 11월 (BRIC 등록일 2020-05-13)
연구진국내 연구진
분야
피인용횟수120회 이상 인용된 논문

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