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서울대학교, 기초과학연구원

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Cell, Published:April 23, 2020 | DOI:https://doi.org/10.1016/j.cell.2020.04.011 The Architecture of SARS-CoV-2 Transcriptome

Dongwan Kim,^1,2 Joo-Yeon Lee,³ Jeong-Sun Yang,³ Jun Won Kim,³ V. Narry Kim,^1,2,4,* and Hyeshik Chang^1,2,*

¹Center for RNA Research, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea
²School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
³Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 28159, Republic of Korea
⁴Lead Contact

^*Correspondence

Abstract

SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3′ tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.

논문정보

TOP52020년 선정

형식Research article
게재일2020년 04월 (BRIC 등록일 2020-04-27)
연구진국내 연구진
분야 생명과학 > 분자생물학
피인용횟수60회 이상 인용된 논문 (상위피인용논문 등록일 2020-10-23)

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