한빛사논문
- 조회2,027
Sei Won Lee1,6, Young Ae Kang2,6, Choong Eun Jin3,6, Ho Cheol Kim1, Geun Su Noh3, Hyo Joo Lee3, Joung Ha Park4, Yong Seo Koo5, Yong Shin3,7 and Sung-Han Kim4,7,*
1Dept of Pulmonary and Critical Care Medicine, Asan Medic1al Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
2Division of Pulmonology, Dept of Internal Medicine, Yonsei University College of Medicine, Severance hospital, Seoul, Republic of Korea
3Dept of Convergence Medicine, Asan Medical Center, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, Seoul, Republic of Korea
4Dept of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
5Dept of Neurology, Asan Medical Center, Seoul, Republic of Korea
6These authors contributed equally to this study as first authors
7These authors contributed equally to this study
*Corresponding author
Abstract
While the Xpert MTB/RIF assay (Cepheid Sunnyvale, CA, USA) has greater than 95% sensitivity for identifying acid-fast bacilli (AFB) smear-positive tuberculosis (TB) cases, for smear-negative pulmonary tuberculosis (PTB) the sensitivity was as low as 60% [1, 2]. To address the low sensitivity of current TB diagnostics for AFB smear-negative cases, we developed a simple and label-free pathogen enrichment using homobifunctional imidoesters (HIs) using a microfluidic (SLIM) platform followed by conventional Mycobacterium tuberculosis PCR to extract low amounts of pathogens from clinical samples [3]. In this study, we compared the diagnostic performance of the SLIM assay with the Xpert MTB/RIF for PTB diagnosis in a country with an intermediate TB burden and a low human immunodeficiency virus burden. The SLIM assay was performed by using 1 mL and 2 mL aliquots of sputum, respectively.
논문정보
- Research letters
- 2020년 03월 (BRIC 등록일 2020-03-27)
- 국내 연구진
- 의약학 > 감염의학
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
