조병관 (KAIST, 지능형바이오시스템설계및합성연구단) | 한빛사논문 > 한빛사

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KAIST, 지능형바이오시스템설계및합성연구단

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Proc. Natl. Acad. Sci. USA, First published March 13, 2020 | https://doi.org/10.1073/pnas.1912289117 Functional cooperation of the glycine synthase-reductase and Wood–Ljungdahl pathways for autotrophic growth of Clostridium drakei

Yoseb Song^a,b, Jin Soo Lee^a,b, Jongoh Shin^a,b, Gyu Min Lee^c, Sangrak Jin^a,b, Seulgi Kang^a,b, Jung-Kul Lee^d, Dong Rip Kim^e, Eun Yeol Lee^f, Sun Chang Kim^a,b,g, Suhyung Cho^a,b, Donghyuk Kim^c,1, and Byung-Kwan Cho^a,b,g,1

^aDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 34141 Daejeon, Republic of Korea;
^bKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, 34141 Daejeon, Republic of Korea;
^cSchool of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, 44919 Ulsan, Republic of Korea;
^dDepartment of Chemical Engineering, Konkuk University, 05029 Seoul,
Republic of Korea;
^eDepartment of Mechanical Engineering, Hanyang University, 04763 Seoul, Republic of Korea;
^fDepartment of Chemical Engineering, Kyung Hee University, 17104 Seoul, Gyeonggi-do, Republic of Korea;
^gIntelligent Synthetic Biology Center, 34141 Daejeon, Republic of Korea

¹To whom correspondence may be addressed.

Abstract

Among CO₂-fixing metabolic pathways in nature, the linear Wood–Ljungdahl pathway (WLP) in phylogenetically diverse acetate-forming acetogens comprises the most energetically efficient pathway, requires the least number of reactions, and converts CO₂ to formate and then into acetyl-CoA. Despite two genes encoding glycine synthase being well-conserved in WLP gene clusters, the functional role of glycine synthase under autotrophic growth conditions has remained uncertain. Here, using the reconstructed genome-scale metabolic model iSL771 based on the completed genome sequence, transcriptomics, ¹³C isotope-based metabolite-tracing experiments, biochemical assays, and heterologous expression of the pathway in another acetogen, we discovered that the WLP and the glycine synthase pathway are functionally interconnected to fix CO₂, subsequently converting CO₂ into acetyl-CoA, acetyl-phosphate, and serine. Moreover, the functional cooperation of the pathways enhances CO₂ consumption and cellular growth rates via bypassing reducing power required reactions for cellular metabolism during autotrophic growth of acetogens.

논문정보

형식Research article
게재일2020년 03월 (BRIC 등록일 2020-03-24)
연구진국내 연구진
분야 생명과학 > 생물공학

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