한빛사논문
- 조회1,712
Jieung Baeka, Younghak Choa, Hyun-Ji Parkb, Goro Choia, Jong Seung Leeb, Minseok Leea, Seung Jung Yua, Seung-Woo Chob,c,d,* Eunjung Leea,* and Sung Gap Ima,*
aDepartment of Chemical and Biomolecular Engineering and KI for NanoCentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
bDepartment of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
cCenter for Nanomedicine, Institute for Basic Science (IBS), Seoul 03772, Republic of Korea
dYonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea
*Corresponding author
J.B., Y.C., and H.-J.P. contributed equally to this work.
Abstract
Cell sheet engineering, a technique utilizing a monolayer cell sheet, has recently emerged as a promising technology for scaffold‐free tissue engineering. In contrast to conventional tissue‐engineering approaches, the cell sheet technology allows cell harvest as a continuous cell sheet with intact extracellular matrix proteins and cell–cell junction, which facilitates cell transplantation without any other artificial biomaterials. A facile, non‐thermoresponsive method is demonstrated for a rapid but highly reliable platform for cell‐sheet engineering. The developed method exploits the precise modulation of cell–substrate interactions by controlling the surface energy of the substrate via a series of functional polymer coatings to enable prompt cell sheet harvesting within 100 s. The engineered surface can trigger an intrinsic cellular response upon the depletion of divalent cations, leading to spontaneous cell sheet detachment under physiological conditions (pH 7.4 and 37 °C) in a non‐thermoresponsive manner. Additionally, the therapeutic potential of the cell sheet is successfully demonstrated by the transplantation of multilayered cell sheets into mouse models of diabetic wounds and ischemia. These findings highlight the ability of the developed surface for non‐thermoresponsive cell sheet engineering to serve as a robust platform for regenerative medicine and provide significant breakthroughs in cell sheet technology.
논문정보
TOP52020년 후보
- Research article
- 2020년 03월 (BRIC 등록일 2020-04-17)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
