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고려대학교, Shenzhen University

Biomaterials, Available online 7 March 2020, 119938 | https://doi.org/10.1016/j.biomaterials.2020.119938 STORM imaging of mitochondrial dynamics using a vicinal-dithiol-proteins-targeted probe

Bingling Chen^a,1, Wanjun Gong^a,1, Zhigang Yang^a,*, Wenhui Pan^a, Peter Verwilst^b, Jinwoo Shin^b, Wei Yan^b, Liwei Liu^a, Junle Qu^a,*, Jong Seung Kim^a,b,*

^aKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China
^bChemistry Department, Korea University, Seoul, 02841, Republic of Korea

¹These authors contributed equally to this work.
^*Corresponding author.

Abstract

Stochastic optical reconstruction microscopy (STORM) is a promising method for the visualization of ultra-fine mitochondrial structures. However, this approach is limited to monitoring dynamic intracellular events owing to its low temporal resolution. We developed a new strategy to capture mitochondrial dynamics using a compressed sensing STORM algorithm following raw data pre-treatments by a noise-corrected principal component analysis and K-factor image factorization. Using STORM microscopy with a vicinal-dithiol-proteins targeting probe, visualizing mitochondrial dynamics was attainable with spatial and temporal resolutions of 45 nm and 0.8 s, notably, dynamic mitochondrial tubulation retraction of ∼746 nm in 1.2 s was monitored. The labeled conjugate was observed as clusters (radii, ∼90 nm) distributed on the outer mitochondrial membranes, not yet reported as far as we know. This strategy is promising for the quantitative analysis of intracellular behaviors below the optical diffraction limit.

논문정보

형식Research article
게재일2020년 03월 (BRIC 등록일 2020-03-16)
연구진국내(교신)+국외 연구진
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