한빛사논문
- 조회1,883
Naresh K. Hanchatea,1, Eun Jeong Leea,1, Andria Ellisb,2, Kunio Kondoha,2,3, Donghui Kuanga, Ryan Basomc, Cole Trapnellb,d, and Linda B. Bucka,d,4
aBasic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; bDepartment of Genome Sciences, University of Washington, Seattle, WA 98115; cGenomics and Bioinformatics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA 98109; and dThe Brotman Baty Institute for Precision Medicine, Seattle, WA 98195
1N.K.H. and E.J.L. contributed equally to this work.
2A.E. and K.K. contributed equally to this work.
3Present address: Department of Homeostatic Regulation, Division of Endocrinology and Metabolism, National Institute for Physiological Sciences, 444-8585 Myodaiji, Okazaki, Aichi, Japan.
4To whom correspondence may be addressed.
Abstract
The mouse brain contains about 75 million neurons interconnected in a vast array of neural circuits. The identities and functions of individual neuronal components of most circuits are undefined. Here we describe a method, termed “Connect-seq,” which combines retrograde viral tracing and single-cell transcriptomics to uncover the molecular identities of upstream neurons in a specific circuit and the signaling molecules they use to communicate. Connect-seq can generate a molecular map that can be superimposed on a neuroanatomical map to permit molecular and genetic interrogation of how the neuronal components of a circuit control its function. Application of this method to hypothalamic neurons controlling physiological responses to fear and stress reveals subsets of upstream neurons that express diverse constellations of signaling molecules and can be distinguished by their anatomical locations.
single-cell RNA-seq, neural circuits, stress
논문정보
- Research article
- 2020년 02월 (BRIC 등록일 2020-02-21)
- 국외 연구진
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