상위피인용논문
- 조회264
서울시립대학교
Nivedita Chatterjee, Hyun-Jeong Eom, Jinhee Choi*
School of Environmental Engineering, Graduate School of Energy and Environmental system Engineering, University of Seoul, 163 Siripdaero, Dongdaemun-gu, Seoul 130-743, Republic of Korea
*Corresponding author : Jinhee Choi
Abstract
The raised considerable concerns about the possible environmental health and safety impacts of graphene nanomaterials and their derivatives originated from their potential widespread applications. We performed a comprehensive study about biological interaction of grapheme nanomaterials, specifically in regard to its differential surface functionalization (oxidation status), by using OMICS in graphene oxide (GO) and reduced graphene oxide (rGO) treated HepG2 cells. Differential surface chemistry (particularly, oxidation – O/C ratio) modulates hydrophobicity/philicity of GO/rGO which in turn governs their biological interaction potentiality. Similar toxic responses (cytotoxicity, DNA damage, oxidative stress) with differential dose dependency were observed for both GO and rGO but they exhibited distinct mechanism, such as, hydrophilic GO showed cellular uptake, NADPH oxidase dependent ROS formation, high deregulation of antioxidant/DNA repair/apoptosis related genes, conversely, hydrophobic rGO was found to mostly adsorbed at cell surface without internalization, ROS generation by physical interaction, poor gene regulation etc. Global gene expression and pathway analysis displayed that TGFβ1 mediated signaling played the central role in GO induced biological/toxicological effect whereas rGO might elicited host-pathogen (viral) interaction and innate immune response through TLR4–NFkB pathway. In brief, the distinct biological and molecular mechanisms of GO/rGO were attributed to their differential surface oxidation status.
Keywords
Graphene oxide (GO); Reduced graphene oxide (rGO); Surface oxidation state; Systems toxicology; TGFβ1 pathway; TLR4–NFkB pathway
논문정보
- Research article
- 2014년 01월 (BRIC 등록일 2020-02-19)
- 국내 연구진
- 120회 이상 인용된 논문
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