한빛사논문
- 조회1,350
Ruri Leea,*, Hae Ju Koa,*, Kimin Kima, Yehjoo Sohna, Seo Yun Minb, Jeong Ah Kimb, Dokyun Nac and Ju Hun Yeona
aDepartment of Integrative Biosciences, University of Brain Education, Cheonan, Republic of Korea; bBiomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea; cSchool of Integrative Engineering, Chung-Ang University, Seoul, Republic of Korea
CONTACT to Ju Hun Yeon
Department of Integrative Bioscience, UBE, Cheonan, Republic of Korea
*These authors contributed equally to this work.
Abstract
Consumer interest in cosmetic industry products that produce whitening effects has increased demand for agents that decrease melanin production. Many such anti-melanogenic agents are associated with side effects, such as contact dermatitis and high toxicity, and also exhibit poor skin penetration. Considerable recent research has focused on plant-derived products as alternatives to chemotherapeutic agents that possess fewer side effects. In the current study, we investigated the anti-melanogenic effects of extracellular vesicles (EVs) extracted from leaves and stems of Dendropanax morbifera. Using spectrophotometric and biochemical approaches, we found that leaf-derived extracellular vesicles (LEVs) and stem-derived extracellular vesicles (SEVs) reduced melanin content and tyrosinase (TYR) activity in the B16BL6 mouse melanoma cell line in a concentration-dependent manner. An electron microscopy analysis further confirmed that LEVs and SEVs induce a concentration-dependent decrease in melanin content in melanoma cells. Both LEVs and SEVs exerted a greater whitening effect on melanoma cells than arbutin, used as a positive control, with LEVs producing the greater effect. Notably, neither LEVs nor SEVs induced significant cytotoxicity. We also examined the effects of plant-derived EVs on the expression of tyrosinase-related proteins (TRPs) in melanoma cells. LEVs inhibited expression of melanogenesis-related genes and proteins, including microphthalmia-associated transcription factor (MITF), TYR, TRP-1 and TRP-2. In a human epidermis model, LEVs exerted a stronger inhibitory effect on melanin production than arbutin. Collectively, our data suggest that LEVs from D. morbifera may be a novel candidate natural substance for use as an anti-melanogenic agent in cosmeceutical formulations.
KEYWORDS: Plant-derived EVs, LEVs and SEVs, anti-melanogenic, TYR activity, melanin content
논문정보
- Research article
- 2019년 12월 (BRIC 등록일 2019-12-31)
- 국내 연구진
- 생명과학 > 생물공학
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