김형준 (국립수산물품질관리원)

구.농수식품

조회839

국립수산물품질관리원

Dev. Comp. Immunol., Available online 9 October 2019, Volume 103, 103518 | https://doi.org/10.1016/j.dci.2019.103518 Involvement of CD4-1 T cells in the cellular immune response of olive flounder (Paralichthys olivaceus) against viral hemorrhagic septicemia virus (VHSV) and nervous necrosis virus (NNV) infection

Abstract

Jae Wook Jung^a, Jung Seok Lee^a, Jaesung Kim^a, Se Pyeong Im^a, Si Won Kim^a, Jassy Mary S. Lazarte^a, Young Rim Kim^a, Jin Hong Chun^a, Min Woo Ha^c, Na Na Kim^d, Kim D. Thompson^e, Hyoung Jun Kim^f,1,*, Tae Sung Jung^a,b,1,*

^a Lab. of Aquatic Animal Diseases, Research Institute of Natural Science, College of Veterinary Medicine, Gyeongsang National University, 501 Jinju, Gyeongnam, 52828, South Korea
^b Centre for Marine Bioproducts Development, Flinders University, Bedford Park, Adelaide, SA, 5042, Australia
^c College of Pharmacy, Gyeongsang National University, 501, Jinju-daero, Jinju, Gyeongnam, 52828, South Korea
^dInland Aquaculture Research Center, NIFS, Changwon, 645-806, South Korea
^e Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, UK
^fLab. of Aquatic Animal Quarantine, General Service Division, National Fishery Products Quality Management Service, Busan 49111, South Korea

¹Both authors contributed equally to this manuscript.

^*Corresponding authors

Abstract
The occurrence of CD4 helper T cells has already been established for a number of teleost species, though, it has not been possible to analyze these responses at a cellular level due to a large lack of appropriate monoclonal antibodies (mAbs). In the present study, we produced a mAb against olive flounder (Paralichthys olivaceus) CD4-1 lymphocyte to investigate the functional activity of the cells to improve our understanding of the T cell response in this species. This mAb is specifically able to detect CD4-1 lymphocytes in olive flounder proved by immunofluorescence staining and RT-PCR analysis. In flow cytometry analysis, the number of CD4-1-positive lymphocytes was observed to gradually increase from 3 days post infection (dpi) and then reach peak at 7 dpi against two viruses challenge. As a conclusion, both the basic properties of CD4-1 T cells and its response to viral infections in olive flounder are very similar to the helper T cells in terrestrial animals.

Keywords : CD4-1 T lymphocytes; Olive flounder; Viral hemorrhagic septicemia virus; Nervous necrosis virus; Helper T cells

논문정보

형식Research article
게재일2019년 10월 (BRIC 등록일 2019-10-30)
연구진국내(교신)+국외 연구진
분야
구분구・농수식품

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