구.농수식품
- 조회778
Abstract
Hahyun Parka,1, Jin-Young Leeb,1, Sunwoo Parka, Gwonhwa Songa,*, Whasun Limc,*
a Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea
b Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA
c Department of Food and Nutrition, Kookmin University, Seoul, 02707, Republic of Korea
1These authors contributed equally to this work.
*Corresponding authors : Gwonhwa Song, Whasun Lim
Abstract
Etoxazole, a chitin synthesis inhibitor, is widely used to control insects and mites by causing developmental defects. Despite the many advantages of pesticides, the inhibitory effects of most pesticides including etoxazole are based on biochemical reaction and their widespread application is considered as a major risk to human health and the environment because of bioaccumulation and non-target toxic effects. Though used in agricultural area, the pesticide residues run off through rivers or ocean, where diverse aquatic organisms live. Since there are no studies evaluating the risks of etoxazole exposure in embryogenesis of aquatic organisms, we investigated the adverse effects of etoxazole on development and angiogenesis in zebrafish embryos, which are considered to be an effective model for detecting ecotoxicological effects of widely used compounds, especially affecting aquatic organisms. Etoxazole induced yolk sac and heart edema, as well as loss of viability, abnormal heart rate, and developmental deficiency. Through a mechanistic approach, we also showed that etoxazole caused reactive oxygen species accumulation, inhibited the expression of cell cycle activating genes, and induced apoptosis. In addition, we investigated effects of etoxazole on cardiovascular development by demonstrating the loss of vascular structure in response to etoxazole exposure in fli1:eGFP transgenic zebrafish model. Collectively, this first assessment demonstrating the effects of etoxazole on embryogenesis and cardiovascular development provides clear evidence for the toxicity of etoxazole and contributes important data towards formulating safety guidelines on the potential hazards of etoxazole for aquatic environment.
Keywords : Zebrafish; Etoxazole; Embryotoxicity; Development; Angiogenesis
논문정보
- Research article
- 2019년 10월 (BRIC 등록일 2019-10-24)
- 국내(교신)+국외 연구진
- 생명과학 > 발생생물학
- 구・농수식품
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