한빛사논문
- 조회863
Se-jeong Kima,b,1, Jaesung Parka,b,1, Eun Mi Kima,b, Jong-Jin Choic, Ha-Na Kimc, Ian L. Chind, Yu Suk Choid, Sung-Hwan Moonc, Heungsoo Shina,b,*
a Department of Bioengineering, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul, 04763, Republic of Korea
b BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul, 04763, Republic of Korea
c Department of Medical Science, School of Medicine, Konkuk University, Seoul, 143-701, Republic of Korea
d School of Human Science, University of Western Australia, Perth, WA, 6009, Australia
1These authors contributed equally to this work.
*Corresponding author : Heungsoo Shin
Abstract
3D culture of stem cells can improve therapeutic effects. However, there is limited research on how to deliver cultured stem cell spheroids to the desired target. Here, we developed lotus seedpod-inspired hydrogel (LoSH) containing microwells for culture and delivery of stem cell spheroids. Human adipose-derived stem cells (hADSCs) inside the square microwells (200 or 400 μm in width with various depths) spontaneously formed spheroids with high viability (94.08 ± 1.56%), and fibronectins conjugated to the hydrogel successfully gripped the spheroids, similar to the funiculus gripping seeds in the lotus seedpod. The spheroids slightly bound to the LoSH surface at 37 °C were detached by the expansion of LoSH at lower temperature of 4 °C. After spheroid formation, LoSH was placed on the target substrate upside-down, expanded at 4 °C for 10 min, and removed from the target. As a result, the spheroids within the microwell were successfully transferred to the target substrate with high transfer efficiency (93.78 ± 2.30%). A delivery of spheroids from LoSH to full-thickness murine skin wound with chimney model showed significant enhancement of the number of SMA-positive vessels at day 21 compared to the group received the same number of spheroids by injection. Together, our findings demonstrate LoSH as a one-step platform that can culture and deliver spheroids to a large target area, which will be useful for various biomedical applications.
Keywords : Nature-inspired materials; Stimuli-responsive hydrogel; Stem cell spheroid; Spheroid delivery; 3D cell culture
논문정보
- Research article
- 2019년 09월 (BRIC 등록일 2019-10-10)
- 국내(교신)+국외 연구진
- 생명과학 > 생물공학
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
