구.농수식품
- 조회1,257
Abstract
Sunwoo Parka,1, Jin-Young Leeb,1, Hahyun Parka, Gwonhwa Songa,*, Whasun Limc,*
a Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea
b Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA
c Department of Food and Nutrition, Kookmin University, Seoul, 02707, Republic of Korea
*Corresponding authors : Gwonhwa Song, Whasun Lim
1These authors contributed equally to this work.
Abstract
Flufenoxuron, a chitin synthesis inhibitor that is widely used in developed countries as an insecticide, is rarely degraded in the environment. In addition to that in insects, flufenoxuron-mediated non-targeted death in organisms such as lizards and bees has been reported. However, the toxic effects of this compound on vascular development during embryogenesis, as well as the underlying mechanism, have not yet been elucidated. In the present study, we assessed abnormal development and cardiovascular damage induced by flufenoxuron in zebrafish embryos. Exposed zebrafish had malformed eyes and pathological characteristics such as heart and yolk sac edema. In accordance with developmental inhibition, cell cycle regulatory genes were dysregulated in zebrafish embryos upon exposure to flufenoxuron. We also discovered that this agent can disrupt vascular formation by interfering with angiogenesis-associated genes including the genes encoding vascular endothelial growth factor Aa (vegfaa), vegfc, fms-related tyrosine kinase 1 (flt1), and flt4 in zebrafish embryos. These anti-angiogenic effects of flufenoxuron were further verified using a well-known angiogenesis model, namely human umbilical vein endothelial cells. In conclusion, our results suggest that flufenoxuron inhibits overall development and angiogenesis during embryogenesis.
Keywords
Flufenoxuron; Zebrafish; Embryotoxicity; Development; Angiogenesis
논문정보
- Research article
- 2019년 09월 (BRIC 등록일 2019-10-17)
- 국내(교신)+국외 연구진
- 생명과학 > 발생생물학
- 구・농수식품
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