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University of California at Berkeley, Lawrence Berkeley National Laboratory

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Nat. Methods, Published 19 August 2019 | DOI https://doi.org/10.1038/s41592-019-0510-z Oblique-plane single-molecule localization microscopy for tissues and small intact animals

Jeongmin Kim^1,2, Michal Wojcik³, Yuan Wang^1,2, Seonah Moon³, Emilia A. Zin⁴, Nadia Marnani⁵, Zachary L. Newman⁵, John G. Flannery^4,5, Ke Xu^3,6,7* and Xiang Zhang^1,2*

¹ NSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA. ² Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. ³Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA. ⁴ Vision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA. ⁵ Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. ⁶Division of Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. ⁷ Chan Zuckerberg Biohub, San Francisco, CA, USA.

^*Correspondence to Ke Xu or Xiang Zhang.

Abstract

Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.

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형식Research article
게재일2019년 08월 (BRIC 등록일 2019-08-29)
연구진국외 연구진
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