Tung Thanh Pham (영남대학교) | 한빛사논문 > 한빛사

한빛사논문

조회672

영남대학교

Biomaterials, Available online 6 August 2019, Volume 221, 119415 | https://doi.org/10.1016/j.biomaterials.2019.119415 Engineering “cell-particle hybrids” of pancreatic islets and bioadhesive FK506-loaded polymeric microspheres for local immunomodulation in xenogeneic islet transplantation

Tiep Tien Nguyen^a,1, Tung Thanh Pham^a,1, Hanh Thuy Nguyen^a, Mahesh Raj Nepal^a, Cao Dai Phung^a, Zhiwei You^a, Nikita Katila^a, Nirmala Tillija Pun^a, Tae Cheon Jeong^a, Dong-Young Choi^a, Pil-Hoon Park^a, Chul Soon Yong^a, Jong Oh Kim^a, Simmyung Yook^b,*, Jee-Heon Jeong^a,*

^aCollege of Pharmacy, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, South Korea
^b College of Pharmacy, Keimyung University, Daegu, 42601, South Korea

¹These authors equally contributed to this work.

^*Corresponding author : Simmyung Yook, Jee-Heon Jeong

Abstract

Host immune response remains an obstacle in cell-replacement therapy for treating type I diabetes. Long-term systemic immunosuppression results in suboptimal efficacy and adverse reactions. Thus, “cell-particle hybrids” of pancreatic islets and tissue-adhesive, polydopamine-coated, FK506-loaded biodegradable microspheres (PD-FK506-MS) were developed to locally modulate the immune response at the transplantation site. Coating of FK506-MS with PD enabled the rapid formation of stable cell-particle hybrids without significant changes in islet viability and functionality. Extremely low quantities of FK506 (approximately 600 ng per recipient) sustainably released from cell-particle hybrids effectively prolonged survival of xenogeneic islet graft. Interestingly, FK506 exhibited extended bioavailability in the grafts but was undetectable in systemic circulation and other tissues. Moreover, mRNA expression of inflammatory cytokines was significantly inhibited in the PD-FK506-MS-containing grafts but not in lymphoid organs. This study presents a promising platform that facilitates the translation of local immunomodulation towards an effective strategy with improved safety profiles for treating type I diabetes.

Keywords : Tissue adhesion; Local immunomodulation; Diabetes; Polydopamine coating

논문정보

형식Research article
게재일2019년 08월 (BRIC 등록일 2019-08-23)
연구진국내 연구진
분야 의약학 > 약학

댓글 작성 로그인

CV 열람하기

연락처 보기