한빛사논문
- 조회2,158
Sung-Jin Park1, Donghui Zhang2,3, Yan Qi2, Yifei Li3,4, Keel Yong Lee1, Vassilios J. Bezzerides3, Pengcheng Yang2, Shutao Xia2, Sean L. Kim1, Xujie Liu3, Fujian Lu3, Francesco S. Pasqualini1, Patrick H. Campbell1, Judith Geva3, Amy E. Roberts3, Andre G. Kleber5, Dominic J. Abrams3, William T. Pu3,6, and Kevin Kit Parker1,3,6,7,*
1 Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA
2 State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, Hubei 430062, China;
3 Department of Cardiology, Boston Children's Hospital, Boston, MA
4 Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China
5 Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA
6 Harvard Stem Cell Institute, Harvard University, Cambridge, MA
7 Sogang-Harvard Research Center for Disease Biophysics, Sogang University, Seoul 121-742, Korea
*Corresponding Author
Abstract
Background: Modeling of human arrhythmias using induced pluripotent stem cell-derived cardiomyocytes has focused on single cell phenotypes. However, arrhythmias are the emergent properties of cells assembled into tissues, and the impact of inherited arrhythmia mutations on tissue-level properties of human heart tissue has not been reported.
Methods: Here, we report an optogenetically-based, human engineered tissue model of catecholaminergic polymorphic ventricular tachycardia (CPVT), an inherited arrhythmia caused by mutation of the cardiac ryanodine channel (RYR2) and triggered by exercise. We developed a hiPSC-CM-based platform to study the tissue-level properties of engineered human myocardium. We investigated pathogenic mechanisms in CPVT, by combining this novel platform with genome editing.
Results: In our model, CPVT tissues were vulnerable to develop reentrant rhythms when stimulated by rapid pacing and catecholamine, recapitulating hallmark features of the disease. These conditions elevated diastolic Ca2+ levels and increased temporal and spatial dispersion of Ca2+ wave speed, creating a vulnerable arrhythmia substrate. Using Cas9 genome editing, we pinpointed a single catecholamine-driven phosphorylation event, RYR2-S2814 phosphorylation by Ca2+-calmodulin-dependent protein kinase II (CaMKII), that is required to unmask the arrhythmic potential of CPVT tissues.
Conclusions: Our study illuminates the molecular and cellular pathogenesis of CPVT and reveals a critical role of CaMKII-dependent reentry in the tissue-scale mechanism of this disease. We anticipate that this approach will be useful to model other inherited and acquired cardiac arrhythmias.
논문정보
- Research article
- 2019년 07월 (BRIC 등록일 2019-08-02)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 의료기기
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