조종수 (서울대학교)

한빛사논문

조회1,196

서울대학교

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Biomaterials, Available online 15 July 2019, Volume 218, 119360 | https://doi.org/10.1016/j.biomaterials.2019.119360 Novel production of natural bacteriocin via internalization of dextran nanoparticles into probiotics

Whee-Soo Kim^a, Geon Goo Han^a, Liang Hong^a, Sang-Kee Kang^b, Mohammadreza Shokouhimehr^c, Yun-Jaie Choi^a,d,*, Chong-Su Cho^a,d,*

^aDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea
^bInstitute of Green-Bio Science & Technology, Seoul National University, Pyeongchang, Gangwon-do 25354, Republic of Korea
^c Department of Materials Science and Engineering, Research Institute of Advanced Materials, Seoul National University, Seoul 08826, Republic of Korea
^d Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

^*Corresponding author : Yun-Jaie Choi, Chong-Su Cho

Abstract

Recently, probiotics has drawn much attention as an alternative of antibiotics because overuse use of antibiotics has caused widespread bacterial resistance. Given that prebiotics enhance the activity of probiotics, we prepared phthalyl dextran nanoparticles (PDNs) by conjugation of phthalic anhydride with dextran to form a prebiotic and checked its effects on the cellular and antimicrobial properties of the probiotics. First, we found that the internalization of PDNs by probiotics was dependent on temperature, time, and glucose transporters. Internalization of PDNs enhanced the production of antimicrobial peptides by probiotics through self-defense mechanism and resulted in higher antimicrobial activities against Gram-positive and -negative pathogens compared to probiotics themselves. Moreover, pediocin produced by PDN-internalized probiotics was able to suppress pathogenic gut infections and alter the population of gut microbiome in vivo. The enhanced antimicrobial property of Pediococcus acidilactidi internalized with PDNs could decrease the number of pathogens and increase beneficial bacteria species in mice. Furthermore, the composition of gut microbiome was changed, and resulted in preventing reduction of the diversity of the microflora. Our results indicate that PDNs as a new type of prebiotic can regulate probiotic bacterial metabolism, suggesting a new avenue for probiotic modulation and their use in addressing the challenge of bacterial resistance.

Keywords : Prebiotics; Probiotics; Dextran nanoparticles; Internalization; Antimicrobial peptide

논문정보

형식Research article
게재일2019년 07월 (BRIC 등록일 2019-12-02)
연구진국내 연구진
분야 생명과학 > 미생물학

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