한빛사논문
- 조회896
POSTECH
Haw-Young Kwona,†, Xiao Liug,†, Eun Gyeong Choib, Jung Yeol Leec, So-Young Choig, Jun-Young Kimd, Lu Wange, Sung-Jin Parkf, Beomsue Kimf, Yong-An Leef, Jong-Jin Kima, Nam Young Kangc,* Young-Tae Changa,b,f,g*
aCenter for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, Gyeongbuk 37673, Korea
bDepartment of Chemistry, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, 117543, Singapore
cNew drug discovery Center, Daegu-Gyeongbuk Medivalley Innovation Foundation (DGMIF), 80 Chumbok-ro, Dong-Gu, Daegu, 41061, Korea
dSL VAXiGEN A-B1, KOREA BIO PARK, Seongnam-si, 134488, Gyenggi-do, Korea
eDepartment of Chemical Biology, Max-Planck-Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
fSingapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, # 02-02 Helios, 138667, Singapore.
gPohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, Korea
†These authors contributed equally.
*To whom correspondence should be addressed.
Abstract
Rapid and sensitive classification of bacteria is the first step for bacterial community research and the treatment of clinical infection. The historic golden standard Gram staining is composed of multi‐step processes and requires experienced technical skill to achieve reliable results, and also the resulting fixed bacteria is not available for further vital study. Herein, we present a novel fluorescent probe BacGO, which shows the best universal selectivity for Gram‐positive bacteria among known probes with minimum staining procedure for sample detection and enrichment of the live bacteria. We also successfully demonstrated BacGO in one‐pot colony staining in plate culture, and an assessment of the Gram status in the bacterial community from wastewater sludge. Furthermore, the high sensitivity and selectivity of probe allowed BacGO for selective Gram‐positive bacterial detection in infection, not only in vitro but also in vivo keratitis mouse model. BacGO provides an unprecedented research tool for dynamic bacterial community study and clinical application.
Keywords : bioimaging, fluorescent probes, Gram-positive bacteria, boronic acid, SAR study
논문정보
- Research article
- 2019년 04월 (BRIC 등록일 2019-05-08)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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