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한국과학기술연구원, UST

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Adv. Sci., First published: 16 April 2019 | https://doi.org/10.1002/advs.201802115 A Novel Blood‐Based Colorectal Cancer Diagnostic Technology Using Electrical Detection of Colon Cancer Secreted Protein‐2

Minhong Jeun^a, Hyo Jeong Lee^b, Sungwook Park^a,c, Eun‐ju Do^d, Jaewon Choi^a,b, You‐Na Sung^e, Seung‐Mo Hong^e, Sang‐Yeob Kim^d,f, Dong‐Hee Kim^d, Ja Young Kang^d, Hye‐Nam Son^d, Jinmyoung Joo^d,f, Eun Mi Song^g, Sung Wook Hwang^g, Sang Hyoung Park^g, Dong‐Hoon Yang^g, Byong Duk Ye^g, Jeong‐Sik Byeon^g, Jaewon Choe^g, Suk‐Kyun Yang^g, Helen Moinova^h, Sanford D. Markowitz^h,i, Kwan Hyi Lee^a,c,*, Seung‐Jae Myung^d,f,g,*

^aCenter for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea
^bHealth Screening & Promotion Center, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea
^cDivision of Bio-Medical Science & Technology, KIST School - Korea University of Science and Technology (UST), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea
^dAsan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea
^eDepartment of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea
^fDepartment of Convergence Medicine, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea
^gDepartment of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea
^hDepartment of Medicine and Case Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH, USA
ⁱUniversity Hospitals Seidman Cancer Center, 10900 Euclid Ave, Cleveland, OH, USA

^*To whom correspondence should be addressed.

M.J, H.J.L, and S.P. contributed equally to this work.

Abstract

Colorectal cancer (CRC) is the second‐leading cause of cancer‐related mortality worldwide, which may be effectively reduced by early screening. Colon cancer secreted protein‐2 (CCSP‐2) is a promising blood marker for CRC. An electric‐field effect colorectal sensor (E‐FECS), an ion‐sensitive field‐effect transistor under dual gate operation with nanostructure is developed, to quantify CCSP‐2 directly from patient blood samples. The sensing performance of the E‐FECS is verified in 7 controls and 7 CRC samples, and it is clinically validated on 30 controls, 30 advanced adenomas, and 81 CRC cases. The concentration of CCSP‐2 is significantly higher in plasma samples from CRC and advanced adenoma compared with controls (both P < 0.001). Sensitivity and specificity for CRC versus controls are 44.4% and 86.7%, respectively (AUC of 0.67), and 43.3% and 86.7%, respectively, for advanced adenomas (AUC of 0.67). CCSP‐2 detects a greater number of CRC cases than carcinoembryonic antigen does (45.6% vs 24.1%), and the combination of the two markers detects an even greater number of cases (53.2%). The E‐FECS system successfully detects CCSP‐2 in a wide range of samples including early stage cancers and advanced adenoma. CCSP‐2 has potential for use as a blood‐based biomarker for CRC.

Keywords: colorectal adenomas, colorectal cancer, colorectal cancer screening, electric-ﬁeld effect colorectal sensor, tumor markers

논문정보

형식Research article
게재일2019년 04월 (BRIC 등록일 2019-04-24)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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