상위피인용논문
성균관대학교
Minh Thu Nguyen1, Seung Phill Choi1, Jinwon Lee2, Jae Hwa Lee3, and Sang Jun Sim1*
1Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea
2Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea
3Department of Pharmaceutical Engineering, Silla University, Pusan 617-736, Korea
*Corresponding author
Abstract
Certain microalgae have been known to use light and various carbon sources to produce carbohydrates, mainly in the form of starch. This is one of the pertinent feedstocks replacing agricultural products for the production of bioethanol by yeast. This study focuses upon dilute acid hydrothermal pretreatments at low cost and high efficiency to compete with current methods, and employs Chlamydomonas reinhardtii UTEX 90 as the feedstock. With dry cells of 5% (w/v), the algal biomass was pretreated with sulfuric acid (1-5%) under temperatures from 100 to 120oC, from 15 to 120 min. As a result, the glucose release from the biomass was maximum at 58% (w/w) after pretreatment with 3% sulfuric acid at 110 degrees for 30 min. This method enabled not only starch, but also the hydrolysis of other oligosaccharides in the algal cell in high efficiency. Arrheniustype of model equation enabled extrapolation of some yields of glucose beyond this range. The pretreated slurry was fermented by yeast, Saccharomyces cerevisiae S288C, resulting in an ethanol yield of 29.2% from algal biomass. This study suggests that the pretreated algal biomass is a suitable feedstock for ethanol production and can have a positive impact on large-scale applied systems.
Keywords: Acid pretreatment, ethanol, microalgae
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