원호근 (중앙백신연구소, 서울대학교)

구.농수식품

조회1,079

원호근Hokeun Won

중앙백신연구소, 서울대학교

Vet. Microbiol., Volume 231, April 2019, Pages 120-128 | https://doi.org/10.1016/j.vetmic.2019.03.009 Assessment of the safety and efficacy of an attenuated live vaccine based on highly virulent genotype 2b porcine epidemic diarrhea virus in nursing piglets

Abstract

Guehwan Jang^a,1, Hokeun Won^b,d,1, Dong-Uk Lee^b, Yun-Hee Noh^b, Seung-Chul Lee^b, Hwan-Won Choi^b, In-Joong Yoon^b, Yoo Jin Lee^c, Han SangYoo^d, Changhee Lee^a,*

^a Animal Virology Laboratory, School of Life Sciences, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea
^b ChoongAng Vaccine Laboratories, Daejeon, 34055, Republic of Korea
^c Department of Neural Development and Disease, Korea Brain Research Institute, Daegu, 41068, Republic of Korea
^d Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea

¹The first two authors contributed equally to this work and share co-first authorship.

^*Corresponding author

Abstract
We have previously reported the generation of the attenuated KNU-141112-S DEL5/ORF3 virus by continuous propagation of highly virulent G2b porcine epidemic diarrhea virus (PEDV) in Vero cells. The present study aimed to assess the safety of S DEL5/ORF3 and to evaluate its effectiveness as a live vaccine for prime-booster vaccinations. Reversion to virulence experiments revealed that the S DEL5/ORF3 strain retains its attenuated phenotype and genetic stability after five successive passages in susceptible piglets. Pregnant sows were primed orally with an S DEL5/ORF3 live vaccine and boosted intramuscularly twice with a commercial killed vaccine at 2-week intervals prior to parturition. This sow vaccination regimen completely protected nursing piglets against virulent G2b challenge, as evidenced by the increase in survival rate from 0% to 100% and the significant reduction in diarrhea intensity, including the amount and duration of PEDV fecal shedding. In addition, despite a 2–3 day period of weight loss in piglets from vaccinated sows after challenge, their daily weight gain was recovered at 7 days post-challenge and became similar to that of unchallenged pigs from unvaccinated sows over the course of the experiment. Furthermore, strong antibody responses to PEDV were verified in the sera and colostrum of immunized sows with the prime-boost treatment and their offspring. Altogether, our data demonstrated that the attenuated S DEL5/ORF3 strain guarantees the safety to host animals with no reversion to virulence and is suitable as an effective primary live vaccine providing durable maternal lactogenic immunity for passive piglet protection.

Keywords : Porcine epidemic diarrhea virus; Live-attenuated vaccine; Reversion to virulence; Safety; Prime-boost

논문정보

형식Research article
게재일2019년 04월 (BRIC 등록일 2019-04-15)
연구진국내 연구진
분야 생명과학 > 수의학
구분구・농수식품

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