구.농수식품
Abstract
Bo-Young Leea,1, Beom-Soon Choib,1, Min-Sub Kima,1, Jun Chul Parka, Chang-Bum Jeonga, Jeonghoon Hana, and Jae-Seong Leea,*
a Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea
b Phyzen Genomics Institute, Seoul 08787, South Korea
*Correspondence : Jae-Seong Lee
1These authors contributed equally to this work.
Abstract
The water flea Daphnia magna is a small planktonic cladoceran. D. magna has been used as a model species for ecotoxicology, as it is sensitive to environmental stressors and environmental changes. Since Daphnia is affected by culture environment and each population/strain has its own ecological and genetic characteristics, its population/strain-based genome information is useful for environmental genomic studies. In this study, we assembled and characterized the genome of D. magna. Using a high-density genetic map of D. magna xinb3, the draft genome was integrated to 10 linkage groups (LGs). The total length of the integrated genome was about 123 Mb with N50=10.1 Mb, and the number of scaffolds was 4,193 including 10 LGs. A total of 15,721 genes were annotated after manual curation. Orthologous genes were characterized in the genome and compared with other genomes of Daphnia. In addition, we identified several defense related genes such as cytochrome P450 (CYP) genes, glutathione S-transferase (GST) genes, and ATP-binding cassette (ABC) genes from the assembled D. magna genome for its potential use in molecular ecotoxicological studies in the freshwater environment. This genomic resource will be helpful to study for a better understanding on molecular mechanism in response to various pollutants.
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