장규환 (경북대학교)

구.농수식품

조회862

경북대학교

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Transbound. Emerg. Dis., First published: 12 January 2019 | https://doi.org/10.1111/tbed.13121 Endemic outbreaks due to the re‐emergence of classical swine fever after accidental introduction of modified live LOM vaccine on Jeju Island, South Korea

Abstract

GuehwanJang¹, Joo-AhKim², Won-Myoung Kang², Hyoung-Seok Yang³, Changnam Park³, Kyongju Jeong³, Sung-Up Moon³, Choi-Kyu Park⁴, Young S. Lyoo⁵, and Changhee Lee^1,*

¹ Animal Virology Laboratory, School of Life Sciences, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, SouthKorea
² Animal Health Division, Jeju Special Self-Governing Province, Jeju 63122, South Korea
³ Veterinary Research Institute, Jeju Special Self-Governing Province, Jeju 63344, SouthKorea
⁴ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, SouthKorea
⁵ College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea

^* Corresponding author

Summary
After the unintentional vaccination of the LOM vaccine strain in 2014, classical swine fever virus (CSFV) reemerged in naive pig herds on Jeju Island, South Korea, which had been a CSF-free region with a non-vaccination policy for a decade. Since the re-emergence, endemic outbreaks of CSFV have occurred in the island, causing enormous damage to provincial pig farms. The present study reports the complete genome sequences and molecular characterization of the LOM-derived field CSFV strains responsible for the current outbreaks on Jeju Island. The emergent Jeju LOM-derived isolates shared 98.9-99.7% and 98.7-99.0% nucleotide sequence identity at the E-gene and whole-genome levels compared to the LOM vaccine strain, respectively. Genetic and phylogenetic analyses indicated that the CSFV field isolates were closest to the LOM strains, but appeared to have undergone substantial evolution. The total number of nucleotide and amino acid differences between the LOM vaccine strain and LOM-derived field isolates ranged from 111 and 28 to 148 and 42. These variations were found to be widely distributed throughout the genome and particularly accumulated in non-structural proteins, which might be associated with the potential for LOM to revert to its original low pathogenic form and subsequent horizontal transmission in Jeju swine herds. These data improve our knowledge regarding safety of the LOM vaccine and inherent risk of reversion to natural virulence in host animals.

Keywords: CSFV; Endemic outbreaks; Jeju Island; LOM-derived fieldstrains; Reversion

논문정보

형식Research article
게재일2019년 01월 (BRIC 등록일 2019-01-28)
연구진국내 연구진
분야 생명과학 > 수의학
구분구・농수식품

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