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Nucleic Acids Res., Published: 23 November 2018 | https://doi.org/10.1093/nar/gky1188 Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae

Myung Sup Kim^†, Kyung Hee Cho^†, Kwang Hyun Park, Jyongsik Jang^* and Ji-Sook Hahn^*

School of Chemical and Biological Engineering, Seoul National University, Institute of Chemical Processes, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea

^*To whom correspondence should be addressed.
Correspondence may also be addressed to Jyongsik Jang.

^†The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.

Abstract

In Saccharomyces cerevisiae, Haa1 and War1 transcription factors are involved in cellular adaptation against hydrophilic weak acids and lipophilic weak acids, respectively. However, it is unclear how these transcription factors are differentially activated depending on the identity of the weak acid. Using a field-effect transistor (FET)-type biosensor based on carbon nanofibers, in the present study we demonstrate that Haa1 and War1 directly bind to various weak acid anions with different affinities. Haa1 is most sensitive to acetate, followed by lactate, whereas War1 is most sensitive to benzoate, followed by sorbate, reflecting their differential activation during weak acid stresses. We show that DNA binding by Haa1 is induced in the presence of acetic acid and that the N-terminal Zn-binding domain is essential for this activity. Acetate binds to the N-terminal 150-residue region, and the transcriptional activation domain is located between amino acid residues 230 and 483. Our data suggest that acetate binding converts an inactive Haa1 to the active form, which is capable of DNA binding and transcriptional activation.

논문정보

형식Research article
게재일2018년 11월 (BRIC 등록일 2018-12-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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