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한빛사논문
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Science, 08 Nov 2018 : eaat0174 | DOI: 10.1126/science.aat0174
Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway
Jeong-Mok Kim1, Ok-Hee Seok1, Shinyeong Ju2,3, Ji-Eun Heo1, Jeonghun Yeom3,4, Da-Som Kim1, Joo-Yeon Yoo1, Alexander Varshavsky5, Cheolju Lee3,4,6,*, Cheol-Sang Hwang1,*
1Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, Republic of Korea.
1Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, Republic of Korea.
2Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
3Center for Theragnosis, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
4Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Republic of Korea.
5Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
6Department of Converging Science and Technology, KHU-KIST, Kyung Hee University, Seoul 02447, Republic of Korea.
*Corresponding author : Cheol-Sang Hwang, Cheolju Lee
*Corresponding author : Cheol-Sang Hwang, Cheolju Lee
Abstract
In bacteria, nascent proteins bear the pretranslationally generated N-terminal (Nt) formyl-methionine (fMet) residue. Nt-fMet of bacterial proteins is a degradation signal, termed fMet/N-degron. In contrast, proteins synthesized by cytosolic ribosomes of eukaryotes were presumed to bear unformylated Nt-Met. Here we found that the yeast formyltransferase Fmt1, although imported into mitochondria, could also produce Nt-formylated proteins in the cytosol. Nt-formylated proteins were strongly up-regulated in stationary phase or upon starvation for specific amino acids. This up-regulation strictly required the Gcn2 kinase, which phosphorylates Fmt1 and mediates its retention in the cytosol. We also found that the Nt-fMet residues of Nt-formylated proteins act as fMet/N-degrons, and identified the Psh1 ubiquitin ligase as the recognition component of this eukaryotic fMet/N-end rule pathway, which destroys Nt-formylated proteins.
논문정보
TOP52018년 선정
- Research article
- 2018년 11월 (BRIC 등록일 2018-11-19)
- 국내(교신)+국외 연구진
- 생명과학 > 생화학
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