이동근 (동아대학교) | 한빛사논문 > 한빛사

한빛사논문

조회2,313

동아대학교

ACS Nano, Publication Date (Web): October 31, 2018 | DOI: 10.1021/acsnano.8b03604 Threshold Rigidity Values for the Asbestos-like Pathogenicity of High-Aspect-Ratio Carbon Nanotubes in a Mouse Pleural Inflammation Model.

Dong-Keun Lee^†, Soyeon Jeon^†, Youngju Han^†, Sung-Hyun Kim^†, Seonghan Lee^†, Il Je Yu^‡, Kyung Seuk Song^§, Aeyeon Kang^∥, Wan Soo Yun^∥, Sung-Min Kang^⊥, Yun Suk Huh^*⊥ , and Wan-Seob Cho^*†

^†Lab of Toxicology, Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, 37, Nakdong-daero 550 beon-gil, Busan 49315, Republic of Korea
^‡ HCTm Co., LTD, 74, Seoicheon-ro 578 beon-gil, Majang-myeon, Icheon-si, Gyeonggi-do 17383, Republic of Korea
^§Korea Environment and Merchandise Testing Institute, 8, Gaetbeol-ro 145 beon-gil, Yeonsu-gu, Incheon 21999, Republic of Korea
^∥ Department of Chemistry, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 16419, Republic of Korea
^⊥ Department of Biological Engineering, Inha University, 100, Inharo, Nam-gu, Incheon 22212, Republic of Korea

^*Corresponding Authors

Abstract

The qualitative and quantitative evaluation of the physicochemical parameters associated with the pathogenicity of high-aspect-ratio nanomaterials is important for comprehensive regulation efforts and safety-by-design approaches. Here, we report quantitative data on the correlations between the rigidity of these nanomaterials and toxicity endpoints in vitro and in vivo. As measured by new ISO standards published in 2017, rigidity shows a strong positive correlation with inflammogenic potential, as indicated by inflammatory cell counts and IL-1β (a biomarker for frustrated phagocytosis) levels in both the acute and chronic phases. In vitro experiments using differentiated THP-1 cells find that only highly rigid multiwalled carbon nanotubes (MWCNTs) and asbestos fibers lead to piercing and frustrated phagocytosis. Thus, this study suggests a bending ratio of 0.97 and a static bending persistence length of 1.08 as threshold rigidity values for asbestos-like pathogenicity. However, additional research using MWCNTs with rigidity values that lie between those of non-inflammogenic (Db = 0.66 and SBPL = 0.87) and inflammogenic fibers (Db = 0.97 and SBPL = 1.09) is required to identify more accurate threshold values, which would be useful for comprehensive regulation and safety-by-design approaches based on MWCNTs.

Keywords: carbon nanofiber; carcinogen; frustrated phagocytosis; high aspect ratio nanomaterial; inflammation; multiwalled carbon nanotube; rigidity

논문정보

형식Research article
게재일2018년 10월 (BRIC 등록일 2018-11-15)
연구진국내 연구진
분야 의약학 > 응용의학

댓글 작성 로그인

CV 열람하기

연락처 보기