김호준 (한국과학기술연구원, University of Illinois at Urbana-Champaign) | 한빛사논문 > 한빛사

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한국과학기술연구원, University of Illinois at Urbana-Champaign

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ACS Nano, Publication Date (Web): August 6, 2018 | DOI: 10.1021/acsnano.8b03770 Microfluidics Synthesis of Gene Silencing Cubosomes

Hojun Kim^†‡ , Jaeuk Sung^†, Yunju Chang^†, Alana Alfeche^†, and Cecilia Leal^*†

^†Materials Science and Engineering Department, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, Illinois 61801, United States

^‡Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea

^*Corresponding Author

Abstract

The success of gene technologies hinges on our ability to engineer superior encapsulation and delivery vectors. Cubosomes are lipid-based nanoparticles where membranes, instead of enveloping into classic liposomes, intertwine into complex arrays of pores well-ordered in a cubic lattice. These complex nanoparticles encapsulate large contents of siRNA compared to a liposomal analogue. Importantly, the membranes that form cubosomes have intrinsic fusogenic properties that promote fast endosomal escape. Despite the great potential, traditional routes of forming cubosomes lead to particle sizes too large to fulfill the state-of-the art requirements of delivery vectors. To overcome this challenge, we utilize a microfluidic nanomanufacturing device to synthesize cubosomes and siRNA-loaded cubosomes, termed cuboplexes. Utilizing cryogenic TEM and small angle X-ray scattering we elucidate the time-resolved mechanisms in which microfluidic devices allow the production of small cubosomes and cuboplexes (75 nm) that outperform commercially available delivery vectors, as well as liposome-based systems.

Keywords: bicontinuous cubic; cuboplexes; cubosomes; lipids; microfluidics; siRNA

논문정보

형식Research article
게재일2018년 08월 (BRIC 등록일 2018-08-22)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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