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Adv. Sci., First published: 30 July 2018 | https://doi.org/10.1002/advs.201800614
Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Choong Eun Jina,b, Bonhan Kooa,b, Tae Yoon Leec, Kyudong Hand, Seok Byung Lime, In Ja Parke,* and Yong Shina,b,*
aDepartment of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
bBiomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
cDepartment of Technology Education and Department of Biomedical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
dDepartment of Nanobiomedical Science & BK21 PLUS NBM, Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea
eDepartment of Colon & Rectal Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
*To whom correspondence may be addressed.
aDepartment of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
bBiomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
cDepartment of Technology Education and Department of Biomedical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
dDepartment of Nanobiomedical Science & BK21 PLUS NBM, Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea
eDepartment of Colon & Rectal Surgery, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, 05505 Seoul, Republic of Korea
*To whom correspondence may be addressed.
Abstract
Cell-free nucleic acids (cfNAs) are emerging diagnostic biomarkers for monitoring the treatment and recurrence of cancers. In particular, the biological role and clinical usefulness of cfNAs obtained from the plasma of patients with various cancers are popular and still intensely explored, yet most studies are limited by technical problems during cfNA isolation. A dimethyl dithiobispropionimidate (DTBP)-based microchannel platform that enables spontaneous cfNA capture in 15 min with minimal cellular background and no requirements for use of bulky instruments is reported first. This platform identified KRAS and BRAF hot-spot mutations following cfDNA isolation from the blood plasma and tissues obtained from 30 colorectal cancer patients. The correlation of mutations between the primary tissues and plasma from the patients was high using this platform with whole genome sequencing compared to the spin-column method. This platform can also be combined with various detection approaches (biooptical sensor, Sanger sequencing, and polymerase chain reaction (PCR)) for rapid, simple, low-cost, and sensitive circulating tumor DNA detection in blood plasma. The efficiency and versatility of this platform in isolating cfNAs from liquid biopsies has applications in cancer treatment and precision medicine.
Keywords : biooptical sensors, cell-free nucleic acids, circulating tumor DNA, liquid biopsy, microfluidics platforms
Keywords : biooptical sensors, cell-free nucleic acids, circulating tumor DNA, liquid biopsy, microfluidics platforms
논문정보
- Research article
- 2018년 07월 (BRIC 등록일 2018-08-10)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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