강훈승 (전남대학교)

상위피인용논문

조회884

전남대학교

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Plant J., First published: 21 March 2007 | https://doi.org/10.1111/j.1365-313X.2007.03057.x Functional characterization of a glycine‐rich RNA‐binding protein 2 in Arabidopsis thaliana under abiotic stress conditions

Joo Y. Kim¹, Su J. Park¹, Boseung Jang², Che-Hun Jung², Sung J. Ahn¹, Chang-Hyo Goh¹, Kyoungwon Cho³, Oksoo Han³ and Hunseung Kang^1,*¹Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute, College of Agriculture and Life Sciences,

²Department of Chemistry, College of Life Science, and

³Department of Genetic Engineering, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757,Korea

^*For correspondence : Hunseung Kang

Abstract

Although glycine-rich RNA?binding protein 2 (GRP2) has been implicated in plant responses to environmental stresses, the function and importance of GRP2 in stress responses are largely unknown. Here, we examined the functional roles of GRP2 in Arabidopsis thaliana under high?salinity, cold or osmotic stress. GRP2 affects seed germination of Arabidopsis plants under salt stress, but does not influence seed germination and seedling growth of Arabidopsis plants under osmotic stress. GRP2 accelerates seed germination and seedling growth in Arabidopsis plants under cold stress, and contributes to enhancement of cold and freezing tolerance in Arabidopsis plants. No differences in germination between the wild?type and transgenic plants were observed following addition of abscisic acid (ABA) or glucose, implying that GRP2 affects germination through an ABA?independent pathway. GRP2 complements the cold sensitivity of an Escherichia coli BX04 mutant and exhibits transcription anti?termination activity, suggesting that it has an RNA chaperone activity during the cold adaptation process. Mitochondrial respiration and catalase and peroxidase activities were affected by expression of mitochondrial?localized GRP2 in Arabidopsis plants under cold stress. Proteome analysis revealed that expression of several mitochondrial-encoded genes was modulated by GRP2 under cold stress. These results provide new evidence indicating that GRP2 plays important roles in seed germination, seedling growth and freezing tolerance of Arabidopsis under stress conditions, and that GRP2 exerts its function by modulating the expression and activity of various classes of genes.

Keywords: abiotic stress, Arabidopsis thaliana, glycine-rich RNA-binding protein, RNA recognition motif,stress adaptation

논문정보

형식Research article
게재일2007년 03월 (BRIC 등록일 2018-06-19)
연구진국내 연구진
분야 생명과학 > 식물학
피인용횟수120회 이상 인용된 논문

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