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Adv. Mater., First published: 18 May 2018 | https://doi.org/10.1002/adma.201706764
Artificial Rod and Cone Photoreceptors with Human‐Like Spectral Sensitivities
Byeongho Park1,2, Heehong Yang3,4, Tai Hwan Ha5, Hyun Seo Park1, Seung Ja Oh1, Yong-Sang Ryu1, Youngho Cho1,6, Hyo-Suk Kim1,7, Juyeong Oh1,2, Dong Kyu Lee1, Chulki Kim1, Taikjin Lee1, Minah Seo1, Jaebin Choi1, Young Min Jhon1, Deok Ha Woo1, Seok Lee1, Seok Hwan Kim8, Hyuk-Jae Lee6, Seong Chan Jun2, Hyun Seok Song9,10,11,*, Tai Hyun Park3,* and Jae Hun Kim1,*
1Korean Institute of Science and Technology, Seoul 02792, Republic of Korea
1Korean Institute of Science and Technology, Seoul 02792, Republic of Korea
2Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea
3School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea
4Protein Engineering Laboratory, Recombinants Unit, MOGAM Institute for Biomedical Research, Yongin 16924, Republic of Korea
5Hazards Monitoring Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34134, Republic of Korea
6College of Science and Technology, Kookmin University, Seoul 02707, Republic of Korea
7Department of Electronics and Communications Engineering, Kwang-woon University, Seoul 01890, Republic of Korea
8Department of Ophthalmology, Seoul National University Boramae Hospital, Seoul 07061, Republic of Korea
9Korea Basic Science Institute (KBSI), Republic of Korea
10Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea
11Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea
B.P. and H.Y. contributed equally to this work.
*To whom correspondence may be addressed.
B.P. and H.Y. contributed equally to this work.
*To whom correspondence may be addressed.
Abstract
Photosensitive materials contain biologically engineered elements and are constructed using delicate techniques, with special attention devoted to efficiency, stability, and biocompatibility. However, to date, no photosensitive material has been developed to replace damaged visual-systems to detect light and transmit the signal to a neuron in the human body. In the current study, artificial nanovesicle-based photosensitive materials are observed to possess the characteristics of photoreceptors similar to the human eye. The materials exhibit considerably effective spectral characteristics according to each pigment. Four photoreceptors originating from the human eye with color-distinguishability are produced in human embryonic kidney (HEK)-293 cells and partially purified in the form of nanovesicles. Under various wavelengths of visible light, electrochemical measurements are performed to analyze the physiological behavior and kinetics of the photoreceptors, with graphene, performing as an electrode, playing an important role in the lipid bilayer deposition and oxygen reduction processes. Four nanovesicles with different photoreceptors, namely, rhodopsin (Rho), short-, medium-, and longwave sensitive opsin 1 (1SW, 1MW, 1LW), show remarkable color-dependent characteristics, consistent with those of natural human retina. With four different light-emitting diodes for functional verification, the photoreceptors embedded in nanovesicles show remarkably specific color sensitivity. This study demonstrates the potential applications of light-activated platforms in biological optoelectronic industries.
논문정보
- Research article
- 2018년 05월 (BRIC 등록일 2018-05-31)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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