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Proc. Natl. Acad. Sci. USA, Published ahead of print May 21, 2018 | https://doi.org/10.1073/pnas.1721241115
Epigenetic switch from repressive to permissive chromatin in response to cold stress
Junghoon Parka,b,1, Chae Jin Lima,b,1, Mingzhe Shenb,1, Hee Jin Parka,c, Joon-Yung Chab, Elisa Iniestod, Vicente Rubiod, Tesfaye Mengistee, Jian-Kang Zhuf, Ray A. Bressanf, Sang Yeol Leeb, Byeong-ha Leeg, Jing Bo Jinh, Jose M. Pardoi, Woe-Yeon Kimb, and Dae-Jin Yuna,2
aDepartment of Biomedical Science and Engineering, Konkuk University, 05029 Seoul, South Korea; bDivision of Applied Life Science (BK21 plus Program), Plant Molecular Biology and Biotechnology Research Center, Institute of Agriculture and Life Science, Gyeongsang National University, 52828 Jinju, Republic of Korea; cInstitute of Glocal Disease Control, Konkuk University, 05029 Seoul, Republic of Korea; dPlant Molecular Genetics Department, Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas, Campus de la Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain; eDepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907; fDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907; gDepartment of Life Science, Sogang University, 04107 Seoul, South Korea; hInstitute of Botany, Chinese Academy of Sciences, 100093 Beijing, China; and iInstitute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas, 41092 Seville, Spain
1J.P., C.J.L., and M.S. contributed equally to this work.
aDepartment of Biomedical Science and Engineering, Konkuk University, 05029 Seoul, South Korea; bDivision of Applied Life Science (BK21 plus Program), Plant Molecular Biology and Biotechnology Research Center, Institute of Agriculture and Life Science, Gyeongsang National University, 52828 Jinju, Republic of Korea; cInstitute of Glocal Disease Control, Konkuk University, 05029 Seoul, Republic of Korea; dPlant Molecular Genetics Department, Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas, Campus de la Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain; eDepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907; fDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907; gDepartment of Life Science, Sogang University, 04107 Seoul, South Korea; hInstitute of Botany, Chinese Academy of Sciences, 100093 Beijing, China; and iInstitute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas, 41092 Seville, Spain
1J.P., C.J.L., and M.S. contributed equally to this work.
2To whom correspondence should be addressed.
Abstract
Switching from repressed to active status in chromatin regulation is part of the critical responses that plants deploy to survive in an ever-changing environment. We previously reported that HOS15, a WD40-repeat protein, is involved in histone deacetylation and cold tolerance in Arabidopsis. However, it remained unknown how HOS15 regulates cold responsive genes to affect cold tolerance. Here, we show that HOS15 interacts with histone deacetylase 2C (HD2C) and both proteins together associate with the promoters of cold-responsive COR genes, COR15A and COR47. Cold induced HD2C degradation is mediated by the CULLIN4 (CUL4)-based E3 ubiquitin ligase complex in which HOS15 acts as a substrate receptor. Interference with the association of HD2C and the COR gene promoters by HOS15 correlates with increased acetylation levels of histone H3. HOS15 also interacts with CBF transcription factors to modulate cold-induced binding to the COR gene promoters. Our results here demonstrate that cold induces HOS15-mediated chromatin modifications by degrading HD2C. This switches the chromatin structure status and facilitates recruitment of CBFs to the COR gene promoters. This is an apparent requirement to acquire cold tolerance.
histone acetylation | derepression | cold stress response | CUL4-based E3 ligase | HOS15
histone acetylation | derepression | cold stress response | CUL4-based E3 ligase | HOS15
논문정보
- Research article
- 2018년 05월 (BRIC 등록일 2018-05-30)
- 국내(교신)+국외 연구진
- 생명과학 > 식물학
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