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Energy Environ. Sci., First published on 08 May 2018 | 10.1039/C8EE00774H
Ultra-Rapid Rates of Water Splitting for Biohydrogen Gas Production through in vitro Artificial Enzymatic Pathways
Eui-Jin Kim,1, † Jae-Eung Kim,1 and Yi-Heng P. Job Zhang 2 *
1Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
1Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
2Current address: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China
† Current address: Department of Life Science and Basic Science Institute, Sogang University, Seoul 04107, Republic of Korea
* Corresponding Author: Y.-H. Zhang
Abstract
Unlocking the potential of the hydrogen economy requires breakthroughs of production, storage, distribution, and infrastructure. Here we demonstrate an in vitro artificial enzymatic pathway that can produce hydrogen at extremely high rates by splitting water energized by carbohydrates (e.g., starch). This fifteen hyperthermophilic enzymes pathway is comprised of ATP-free starch phosphorylation, an NAD-based pentose phosphate pathway, and a biomimetic electron transport chain consisting of a diaphorase, an electron mediator benzyl viologen (BV), and a [NiFe]-hydrogenase, whereas fast electron transfer was facilitated by utilizing a BV-conjugated diaphorase. The highest reaction rate of 530 mmol H2/L/h was accomplished at 80°C. Two NAD-conjugated dehydrogenases were further applied to enable nine-day hydrogen production with a total turnover number of NAD of over 100,000, along with hyperthermophilic enzymes. This biohydrogen production system characterized in the highest chemical-energy efficiency and exceptionally-high reaction rate addresses challenges associated with cost-effective, distributed hydrogen production, off-board hydrogen storage, and infrastructure.
논문정보
- Research article
- 2018년 05월 (BRIC 등록일 2018-05-17)
- 국외 연구진
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