김대수 (KAIST) | 한빛사논문 > 한빛사

한빛사논문

조회1,483

KAIST

CV File 681 kb

Nano Energy, Volume 44, February 2018, Pages 447-455 | https://doi.org/10.1016/j.nanoen.2017.12.011 Optogenetic control of body movements via flexible vertical light-emitting diodes on brain surface

Seung Hyun Lee^a,1, Jeongjin Kim^b,d,1, Jung Ho Shin^a,1, Han Eol Lee^a, Il-Suk Kang^c, Kiuk Gwak^e, Dae-Shik Kim^e, Daesoo Kim^b,*, Keon Jae Lee^a,*

^aDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
^bDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
^cNational Nanofab Center, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
^dCenter for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea
^eDepartment of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea

¹These authors contributed equally to this work.
^*Corresponding authors.

Abstract

The microstimulation of specific neural populations of the brain is one of the facile and reliable methods used in neuroscience for deduction of functional movement, complex behavior and even long-range connectivity. Recent advanced biomedical tools now employ flexible optoelectronic devices combined with optogenetic mouse models to induce high spatiotemporal modulation of specific brain activity. However, most current applications are limited to activation of small functional regions using blue-light driven channelrhodopsin. In this report, we introduce flexible AlGaInP vertical light-emitting diodes (VLEDs) for perturbation of specific functional areas of mouse cortex. Micro-scaled LEDs effectively compress the conductive balls dispersed in anisotropic conductive film (ACF) resulting red light emissions with high optical power density, capable of stimulating motor neurons deep below layer III from the brain surface. Selective operation of pulsed red light from f-VLEDs induces mouse body movements and synchronized electromyogram (EMG) signals. The expression of chrimson, red-shifted channelrhodopsin, enables red-light excitation of targeted functional area of motor cortex. This demonstration opens new opportunities for entire cortical mapping, to explore the connectivity between undefined motor areas in the mouse brain.

논문정보

형식Research article
게재일2018년 02월 (BRIC 등록일 2020-05-28)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기