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Angew. Chem.-Int. Edit., Version of Record online: 25 JAN 2018 | DOI: 10.1002/anie.201711629 Autophagy Caught in the Act: A Supramolecular FRET Pair Based on an Ultrastable Synthetic Host–Guest Complex Visualizes Autophagosome–Lysosome Fusion

Dr. Meng Li^1,†, Ara Lee^2,†, Dr. Kyung Lock Kim¹, Dr. James Murray¹, Dr. Annadka Shrinidhi¹, Gihyun Sung², Prof. Dr. Kyeng Min Park^1,* and Prof. Dr. Kimoon Kim^1,2,3,4,*¹Center for Self-Assembly and Complexity (CSC), Institute for Basic Science (IBS), Pohang, Republic of Korea

²Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea

³Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea

⁴Department of Nanomaterials Science and Engineering, University of Science and Technology (UST), Daejeon, Republic of Korea
^† These authors contributed equally.

^*Corresponding authors

Abstract

A supramolecular FRET pair based on the ultrahigh binding affinity between cyanine 3 conjugated cucurbit[7]uril (CB[7]-Cy3) and cyanine?5 conjugated adamantylamine (AdA-Cy5) was exploited as a new synthetic tool for imaging cellular processes in live cells. Confocal laser scanning microscopy revealed that CB[7]-Cy3 and AdA-Cy5 were intracellularly translocated and accumulated in lysosomes and mitochondria, respectively. CB[7]-Cy3 and AdA-Cy5 then formed a host?guest complex, reported by a FRET signal, as a result of the fusion of lysosomes and mitochondria. This observation not only indicated that CB[7] forms a stable complex with AdA in a live cell, but also suggested that this FRET pair can visualize dynamic organelle fusion processes, such as those involved in the degradation of mitochondria through autophagy (mitophagy), by virtue of its small size, chemical stability, and ease of use.

Keywords : autophagy;cucurbiturils;FRET;organelle fusion;supramolecular chemistry

논문정보

형식Research article
게재일2018년 01월 (BRIC 등록일 2018-02-12)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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